RT info:eu-repo/semantics/article
T1 Using aequorin probes to measure Ca2+ in intracellular organelles
A1 Alonso Alonso, María Teresa
A1 Rodríguez Prados, Macarena
A1 Navas Navarro, Paloma
A1 Rojo Ruiz, Jonathan
A1 García-Sancho Martín, Francisco Javier
AB Aequorins are excellent tools for measuring intra-organellar Ca2+ and assessing its role in physiological and pathological functions. Here we review targeting strategies to express aequorins in various organelles. We address critical topics such as probe affinity tuning as well as normalization and calibration of the signal. We also focus on bioluminescent Ca2+ imaging in nucleus or mitochondria of living cells. Finally, recent advances with a new chimeric GFP-aequorin protein (GAP), which can be used either as luminescent or fluorescent Ca2+ probe, are presented. GAP is robustly expressed in transgenic flies and mice, where it has proven to be a suitable Ca2+ indicator for monitoring physiological Ca2+ signaling ex vivo and in vivo.
PB Elsevier
YR 2017
FD 2017
LK http://uvadoc.uva.es/handle/10324/35194
UL http://uvadoc.uva.es/handle/10324/35194
LA eng
NO Cell Calcium Volume 64, 2017, Pages 3-11
NO Producción Científica
DS UVaDOC
RD 19-may-2024