RT info:eu-repo/semantics/article
T1 Vertical transmission of Fusarium circinatum mitoviruses FcMV1 and FcMV2-2 via microconidia
A1 Romeralo Tapia, Carmen
A1 Bezos García, Diana
A1 Martínez Álvarez, Pablo
A1 Díez Casero, Julio Javier
K1 Pine pitch canker disease
K1 Chancro resinoso del pino
K1 Hypovirulence
K1 Hipovirulencia
K1 Microconidia
K1 Microconidias
K1 Biological control
K1 Control biológico
K1 3106 Ciencia Forestal
AB Pine Pitch Canker disease, caused by the pathogenic fungus Fusarium circinatum, affectsconifer species worldwide. However, the virulence of the pathogen may be affected by the presenceof mycoviruses. The aim of this laboratory-based study was to investigate the probability and rateof transmission of F. circinatum mitoviruses FcMV1 and FcMV2-2 via microconidia. Ten isolates ofmitovirus-infected F. circinatum were subcultured to produce a total of 100 single-spore colonies(ten replicates per isolate). The total RNA and cDNA obtained from each spore isolate (monosporicculture) were amplified by PCR with specific primers for detection of F. circinatum mitoviruses FcMV1and FcMV2-2. The mitoviruses were detected in a high percentage of the individual spore isolates(between 60% and 100% depending on the fungal isolate). However, the probability of transmissionwas not statistically significantly associated with either the F. circinatum isolate or the viral strain.A high proportion of transmission via microconidia is critical for development of a biological controlprogram against Pine Pitch Canker (PPC) disease in forests. However, further studies are needed toestablish the effect of these mitoviruses on the virulence of F. circinatum.
PB MDPI
SN 1999-4907
YR 2018
FD 2018
LK http://uvadoc.uva.es/handle/10324/40819
UL http://uvadoc.uva.es/handle/10324/40819
LA eng
NO Forests, 2018, vol. 9, n. 6. 10 p.
NO Producción Científica
DS UVaDOC
RD 06-jun-2024