RT info:eu-repo/semantics/article
T1 Assessment of the corneal collagen organization after chemical burn using second harmonic generation microscopy
A1 Bueno, Juan M.
A1 Ávila, Francisco J.
A1 Lorenzo Martín, Elvira
A1 Gallego Muñoz, Patricia
A1 Martínez García, María del Carmen
K1 Córnea - cirugía
K1 Colágeno corneal
K1 Corneal collagen
K1 2407 Biología Celular
AB The organization of the corneal stoma is modified due to different factors, including pathology, surgery or external damage. Here the changes in the organization of the corneal collagen fibers during natural healing after chemical burn are investigated using second harmonic generation (SHG) imaging. Moreover, the structure tensor (ST) was used as an objective tool for morphological analyses at different time points after burn (up to 6 months). Unlike control corneas that showed a regular distribution, the collagen pattern at 1 month of burn presented a non-organized arrangement. SHG signal levels noticeably decreased and individual fibers were hardly visible. Over time, the healing process led to a progressive re-organization of the fibers that could be quantified through the ST. At 6 months, the stroma distribution reached values similar to those of control eyes and a dominant direction of the fibers re-appeared. The present results show that SHG microscopy imaging combined with the ST method is able to objectively monitor the temporal regeneration of the corneal organization after chemical burn. Future implementations of this approach into clinically adapted devices would help to diagnose and quantify corneal changes, not only due to chemical damages, but also as a result of disease or surgical procedures.
PB Optical Society of America (OSA)
SN 2156-7085
YR 2021
FD 2021
LK http://uvadoc.uva.es/handle/10324/45227
UL http://uvadoc.uva.es/handle/10324/45227
LA eng
NO Biomedical Optics Express Vol. 12, Issue 2, pp. 756-765 (2021)
NO Producción Científica
DS UVaDOC
RD 27-abr-2024