RT info:eu-repo/semantics/article
T1 A pilot proteomic study of normal human tears: leptin as a potential biomarker of metabolic disorders
A1 Byambajav, Mungunshur
A1 Arroyo del Arroyo, Cristina
A1 Enriquez De Salamanca Aladro, Amalia
A1 Fernández Martínez, Itziar
A1 Martin, Eilidh
A1 Hagan, Suzanne
K1 Ophthalmology
K1 Endocrinology
K1 Biomarker
K1 Ocular surface
K1 Metabolic protein
K1 Tear fluid
K1 Biomarcador
K1 Superficie ocular
K1 Proteína metabólica
K1 Líquido lagrimal
K1 3201.09 Oftalmología
AB The concentrations of insulin, leptin, active ghrelin, C-peptide and gastric inhibitory polypeptide (GIP) and their inter-day variations were examined in normal human tears. In addition, correlations between the concentrations of these metabolic proteins and ocular surface parameters were determined. Subjects with healthy ocular surfaces attended three visits, with 7-day intervals. Tear evaporation rate (TER) and non-invasive tear break-up time (NITBUT) were assessed, and a total of 2 µL tears were collected from all subjects. Tear fluid concentrations of insulin, leptin, active ghrelin, C-peptide and GIP were measured by multiplex bead analysis. Insulin was the most highly expressed metabolic protein, followed by leptin, C-peptide, active ghrelin and GIP. Of these, only active ghrelin had a significant inter-day variation (p < 0.05). There was no inter-day variation in the mean concentrations of the other metabolic proteins. Leptin had a strong intra-class reproducibility. No correlation was detected between tear metabolic protein concentrations and ocular surface parameters. This pilot study shows, for the first time, that active ghrelin and GIP are detectable in healthy tears. The strong intra-class reproducibility for leptin shows that it could be used as a potential tear fluid biomarker and, possibly, in determining the effects of metabolic disorders on the ocular surface.
PB MDPI
YR 2021
FD 2021
LK https://uvadoc.uva.es/handle/10324/59547
UL https://uvadoc.uva.es/handle/10324/59547
LA eng
NO Applied Sciences, 2021, vol. 11, n. 12, 5755
NO Producción Científica
DS UVaDOC
RD 19-may-2024