RT info:eu-repo/semantics/article
T1 Study of different chiral columns for the enantiomeric separation of azoles using supercritical fluid chromatography
A1 Toribio Recio, Laura
A1 Magdaleno de la Fuente, Irene
A1 Martín Gómez, Beatriz
A1 Martín Gómez, María Teresa
A1 Valverde Bastardo, Silvia
A1 Ares Sacristán, Ana María
K1 Enantiomers - Separation
K1 Pharmacology
K1 Supercritical fluid chromatography
K1 Cromatografía en fase líquida
K1 Antifungal agents - Therapeutic use
K1 Fungicidas
K1 Chromatographic analysis
K1 Chiral drugs - Separation
K1 Separation (Technology)
K1 Separación (Tecnología)
K1 2301 Química Analítica
K1 3209 Farmacología
AB The enantiomeric separation of antifungal compounds is an arduous task in pharmaceutical and biomedical fields due to the different properties that each diastereoisomer presents. The enantioseparation of a group of fungicides (sulconazole, bifonazole, triadimefon and triadimenol) using supercritical fluid chromatography was achieved in this work. For this goal, four different chiral columns based on polysaccharide derivatives, as well as the effect of different chromatographic parameters such as temperature, type and percentage of organic modifier (methanol, ethanol and isopropanol), were thoroughly investigated. The inversion of the elution order of enantiomers as a result of a change in the stationary phase or organic modifier was also evaluated by employing a circular dichroism detector. The best separation conditions, in terms of the enantioresolution and analysis time, were obtained with the Lux® Cellulose-2 column using isopropanol as the organic modifier.
PB MDPI
YR 2023
FD 2023
LK https://uvadoc.uva.es/handle/10324/60670
UL https://uvadoc.uva.es/handle/10324/60670
LA eng
NO Separations, 2023, Vol. 10, Nº. 1, 9
NO Producción Científica
DS UVaDOC
RD 23-may-2024