RT info:eu-repo/semantics/article
T1 Continuous polyhydroxybutyrate production from biogas in an innovative two‐stage bioreactor configuration
A1 Rodríguez Muñoz, Yadira
A1 García, Silvia
A1 Lebrero Fernández, Raquel
A1 Muñoz Torre, Raúl
K1 Biogas biorefinery
K1 Biopolymers
K1 Methane oxidation
K1 Polyhydroxyalkanoate production
K1 23 Química
K1 2391 Química Ambiental
AB Biogas biorefineries have opened up new horizons beyond heat and electricityproduction in the anaerobic digestion sector. Added‐value products such aspolyhydroxyalkanoates (PHAs), which are environmentally benign and potentialcandidates to replace conventional plastics, can be generated from biogas. Thiswork investigated the potential of an innovative two‐stage growth‐accumulationsystem for the continuous production of biogas‐based polyhydroxybutyrate (PHB)usingMethylocystis hirsutaCSC1 as cell factory. The system comprised twoturbulent bioreactors in series to enhance methane and oxygen mass transfer: acontinuous stirred tank reactor (CSTR) and a bubble column bioreactor (BCB) withinternal gas recirculation. The CSTR was devoted to methanotrophic growth undernitrogen balanced growth conditions and the BCB targeted PHB productionunder nitrogen limiting conditions. Two different operational approaches underdifferent nitrogen loading rates and dilution rates were investigated. A balancednitrogen loading rate along with a dilution rate (D) of 0.3 day−1resulted in the moststable operating conditions and a PHB productivity of ~53 g PHB m−3day−1.However, higher PHB productivities (~127 g PHB m−3day−1)wereachievedusingnitrogen excess at a D = 0.2 day−1. Overall, the high PHB contents (up to 48% w/w)obtained in the CSTR under theoretically nutrient balanced conditions and the poorprocess stability challenged the hypothetical advantages conferred by multistagevs single‐stage process configurations for long‐term PHB production.
PB Wiley
SN 0006-3592
YR 2023
FD 2023
LK https://uvadoc.uva.es/handle/10324/61211
UL https://uvadoc.uva.es/handle/10324/61211
LA eng
NO Biotechnology and Bioengineering, 2023.
NO Producción Científica
DS UVaDOC
RD 17-may-2024