RT info:eu-repo/semantics/article T1 Optimization of a simultaneous enzymatic hydrolysis to obtain a high-glucose slurry from bread waste A1 Sigüenza Andrés, Teresa A1 Pando Fernández, Valentín A1 Gómez Pallarés, Manuel A1 Rodríguez Nogales, José Manuel K1 Bread K1 Pan K1 Alimentos - Procesos industriales K1 Saccharification K1 Reducing sugars K1 Glucose K1 Glucosa K1 Hydrolysis K1 Hidrólisis K1 Amylases K1 Enzymes K1 Enzimas - Aplicaciones industriales K1 Sustainable development K1 Desarrollo sostenible K1 3309 Tecnología de Los Alimentos AB Bread and bakery products are among the most discarded food products in the world. This work aims to investigate the potential use of wasted bread to obtain a high-glucose slurry. Simultaneous hydrolysis of wasted bread using α-amylase and glucoamylase was carried out performing liquefaction and saccharification at the same time. This process was compared with a traditional sequential hydrolysis. Temperature and pH conditions were optimized using a response surface design determining viscosity, reducing sugars and glucose concentration during the enzymatic processes. The optimal conditions of pH and temperature in the saccharification stage and the simultaneous hydrolysis were pretty similar. Results show that the slurry produced with simultaneous process had a similar glucose yield at 2 h, and at 4 h a yield higher than that obtained by the sequential method of 4 h and could reduce time and energy. PB MDPI SN 2304-8158 YR 2022 FD 2022 LK https://uvadoc.uva.es/handle/10324/61876 UL https://uvadoc.uva.es/handle/10324/61876 LA eng NO Foods, 2022, Vol. 11, Nº. 12, 1793 NO Producción Científica DS UVaDOC RD 20-may-2024