RT info:eu-repo/semantics/article
T1 Lipin-1-derived diacylglycerol activates intracellular TRPC3 which is critical for inflammatory signaling
A1 Casas Requena, Javier
A1 Meana González, Clara
A1 López López, José Ramón
A1 Balsinde Rodríguez, Jesús
A1 Balboa García, María Ángeles
AB Exposure to Gram-negative bacterial LPS exacerbates host immune responses and may lead to sepsis, a life-threatening condition. Despite its high mortality and morbidity, no drugs specifically directed to treating sepsis are currently available. Using human cell genetic depletion, pharmacological inhibition, live-cell microscopy and organelle-targeted molecular sensors we present evidence that the channel TRPC3 is activated intracellularly during macrophage exposure to LPS and is essential for Ca2+ release from internal stores. In this manner, TRPC3 participates in cytosolic Ca2+ elevations, activation of the transcription factor NF-κB and cytokine upregulation. We also report that TRPC3 is activated by diacylglycerol generated by the phosphatidic acid phosphatase lipin-1. In accord with this, lipin-1-deficient cells exhibit reduced Ca2+ responses to LPS challenge. Finally, pharmacological inhibition of TRPC3 reduces systemic inflammation induced by LPS in mice. Collectively, our study unveils a central component of LPS-triggered Ca2+ signaling that involves intracellular sensing of lipin-1-derived DAG by TRPC3, and opens new opportunities for the development of strategies to treat LPS-driven inflammation.
SN 1420-682X
YR 2021
FD 2021
LK https://uvadoc.uva.es/handle/10324/64305
UL https://uvadoc.uva.es/handle/10324/64305
LA eng
NO Cell Mol Life Sci 1–18. https://doi.org/10.1007/s00018-021-03999-0
NO Producción Científica
DS UVaDOC
RD 24-may-2024