RT info:eu-repo/semantics/article
T1 Improved ocular delivery of quercetin and resveratrol: A comparative study between binary and ternary cyclodextrin complexes
A1 Krstić, Luna
A1 Jarho, Pekka
A1 Ruponen, Marika
A1 Urtti, Arto
A1 González García, María Jesús
A1 Diebold Luque, María Yolanda
K1 Oftalmología
K1 quercetin
K1 resveratrol
K1 Dry eye disease
K1 Cyclodextrins
K1 Quercetin
K1 Enfermedad del ojo seco
K1 Ciclodextrinas
K1 Quercetina
K1 3201.09 Oftalmología
AB The number of patients affected by Dry Eye Disease (DED) had notably increased worldwide, addressing the need of novel therapeutic approaches. Polyphenols, quercetin (QUE) and resveratrol (RSV) show necessary antioxidant and anti-inflammatory properties to manage DED, but their application as topical eyedrops is restricted by low aqueous solubility and low chemical stability. Cyclodextrins (CD) are widely used to improve physicochemical characteristics of drugs. Consequently, the aim of this study was to make a comparison between binary complexes with quercetin, resveratrol and cyclodextrins and tertiary complexes adding hyaluronic acid (HA). Both complexes were able to enhance solubility and stability of QUE and RSV. AFM imaging and DLS measurements disclose the formation of spherical nanoaggregates within tertiary complexes of both QUE and RSV with mean diameters of 103 and 82 nm. Neither complex demonstrated cytotoxic effect in in vitro studies in corneal (HCE) and conjunctival (IM-ConjEpi) cell lines. In HCE cells, complexes containing QUE or RSV at their highest concentrations were able to scavenge more than 95 % of the ROS that were produced intracellularly (p < 0.005). Similar response was observed with IM-ConjEpi cells. The antioxidant effect was maintained in the complexes with HA. This confirmed their potential as viable topical treatment for DED
PB Elsevier
SN 0378-5173
YR 2022
FD 2022
LK https://uvadoc.uva.es/handle/10324/64818
UL https://uvadoc.uva.es/handle/10324/64818
LA eng
NO International Journal of Pharmaceutics, 2022, vol. 624, 122028
NO Producción Científica
DS UVaDOC
RD 10-jun-2024