RT info:eu-repo/semantics/article T1 Assessing the influence of CH 4 concentration during culture enrichment on the biodegradation kinetics and population structure A1 López, Juan C. A1 Quijano, Guillermo A1 Pérez, Rebeca A1 Muñoz Torre, Raúl AB Methanotrophic communities were enriched in three stirred tank reactors continuously supplied with CH4-laden air at 20, 2 and 0.2 gCH4 m−3 in order to evaluate the influence of CH4 concentration on the biodegradation kinetics, population structure and potential polyhydroxyalkanoate production under sequential nitrogen limitations. The population structure of the enriched cultures, dominated by type I methanotrophs, was influenced by CH4 concentration. No significant correlation between CH4 concentration and the maximum specific degradation rate (qmax) or the half-saturation constant (KS) was recorded, microorganisms enriched at 2 gCH4 m−3 presenting the highest qmax and those enriched at 20 and 0.2 gCH4 m−3 exhibiting the lowest KS. Maximum polyhydroxybutyrate (PHB) contents of 1.0% and 12.6% (w/w) were achieved at 20 and 2 g CH4 m−3, respectively. Polyhydroxyvalerate (PHV) was also detected at PHV:PHB ratios of up to 12:1 and 4:1 in the communities enriched at 20 and 0.2 gCH4 m−3, respectively. SN 0301-4797 YR 2014 FD 2014 LK https://uvadoc.uva.es/handle/10324/64830 UL https://uvadoc.uva.es/handle/10324/64830 LA spa NO Volume 146, 15 December 2014, Pages 116-123 DS UVaDOC RD 20-may-2024