Show simple item record

dc.contributor.authorLópez Linares, Juan Carlos 
dc.contributor.authorArnáiz, Esther
dc.contributor.authorMerchán Catalina, Laura
dc.contributor.authorLebrero Fernández, Raquel 
dc.contributor.authorMuñoz Torre, Raúl 
dc.date.accessioned2018-06-13T12:52:26Z
dc.date.issued2018
dc.identifier.citationChemical Engineering Journal, Volume 333, 2018, Pages 529-536es
dc.identifier.urihttp://uvadoc.uva.es/handle/10324/30103
dc.descriptionProducción Científicaes
dc.description.abstractThe potential of biogas (with and without H2S) and volatile fatty acids (VFAs) to support microbial growth and accumulation of polyhydroxyalkanoates (PHAs) in type II methanotrophs was evaluated batchwise under aerobic conditions. Methylocystis hirsuta was able to grow on artificial biogas (70% CH4, 29.5% CO2, 0.5% H2S) and accumulate PHA up to 45 ± 1% (wt.%) under N-limited conditions. The presence of CO2 and H2S did not significantly influence the growth and PHA synthesis in M. hirsuta compared to control tests provided with pure CH4 at similar concentrations. Likewise, the addition of VFAs to the cultivation broth at initial concentrations of 100–200 mg L−1 did not hamper the growth of this strain on artificial biogas. Indeed, the addition of 10% extra carbon in the form of individual VFAs resulted in an increase in the maximum PHA yield and final PHA content up to 0.45–0.63 gPHA gSubstrate 1 and 48–54% (wt.%), respectively, at the expense of a higher energy demand. Valeric acid supplementation supported the highest 3-hydroxyvalerate content (13.5%) within the biocomposite. In this context, this study demonstrated for the first time that 3-hydroxyvalerate synthesis by M. hirsuta did not depend on CH4 assimilation.es
dc.format.mimetypeapplication/pdfes
dc.language.isoenges
dc.publisherElsevieres
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.classificationBiorefineríaes
dc.subject.classificationMetanoes
dc.subject.classificationbio-refineryes
dc.subject.classificationMethanees
dc.titleBiogas-based polyhydroxyalkanoates production by Methylocystis hirsuta: A step further in anaerobic digestion biorefinerieses
dc.typeinfo:eu-repo/semantics/articlees
dc.identifier.doihttps://doi.org/10.1016/j.cej.2017.09.185es
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S138589471731690Xes
dc.peerreviewedSIes
dc.description.embargo2019-12-12es
dc.description.lift2019-12-12
dc.description.projectMinisterio de Economía, Industria y Competitividad (Proyect CTM2015-70442-R and Red NOVEDAR)es
dc.description.projectJunta de Castilla y León (UIC71)es
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record