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dc.contributor.authorLeón, Alberto José
dc.contributor.authorGarrote Adrados, José Antonio 
dc.contributor.authorArranz, Eduardo
dc.date.accessioned2014-02-14T14:09:16Z
dc.date.available2014-02-14T14:09:16Z
dc.date.issued2006
dc.identifier.citationMedicina Clínica, vol.127 n. 4 p.145-152es
dc.identifier.urihttp://uvadoc.uva.es/handle/10324/4330
dc.descriptionProducción Científicaes
dc.description.abstractDietary gluten induces an early response in the intestine of coeliac disease patients (CD), within a few hours, and this is driven by high levels of proinflammatory cytokines, including IFNg and IL-15, as has been thoroughly shown by gluten stimulation of biopsy explants. Our aim was to identify the immune mediators involved in the long-standing inflammation in untreated CD patients at diagnosis. mRNA and protein levels of TNFa, IL-12(p35), IL-12(p40), IL-15, IL-18 and IL-23(p19) were quantified in biopsies from active CD patients, CD patients on a gluten-free diet (GFD), healthy controls, and patients with non-CD inflammation and mild histological changes in the intestine. Biopsies from CD patients on a GFD were also stimulated in vitro with gliadin, and protein expression of IL-15 and IL-18 was analysed. Levels of IL-12 and IL-23 mRNA are nearly absent, and TNFa levels remain unchanged among different groups. Both the active and inactive forms of IL-18 protein have been found in all samples from active CD, and protein expression was only localized within the crypts. Levels of IL-15 mRNA remain unchanged, and protein expression, localized within the lamina propria, is found in a small number of samples. In vitro stimulation with gluten induces the expression of IL-15 and IL-18. In active CD, the early response following gluten intake characterized by high IFNg levels is driven by IL-18, and probably IL-15, and this alternates with periods of long-standing inflammation with moderate IFNg levels, maintained by IL-18 alone.es
dc.format.mimetypeapplication/pdfes
dc.language.isospaes
dc.publisherDoymaes
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectEnfermedad inflamatoria intestinales
dc.subjectCitocinases
dc.titleCitocinas en la patogenia de la enfermedad inflamatoria intestinales
dc.typeinfo:eu-repo/semantics/articlees
dc.relation.publisherversionhttp://www.doyma.eses
dc.identifier.publicationfirstpage145es
dc.identifier.publicationissue4es
dc.identifier.publicationlastpage152es
dc.identifier.publicationtitleMedicina clínicaes
dc.identifier.publicationvolume127es
dc.peerreviewedSIes
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International


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