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dc.contributor.authorAlonso, Andrés
dc.contributor.authorBayón Prieto, Yolanda 
dc.contributor.authorRenedo, Marta
dc.contributor.authorSánchez Crespo, Mariano
dc.date.accessioned2021-07-19T06:06:20Z
dc.date.available2021-07-19T06:06:20Z
dc.date.issued2000
dc.identifier.citationInternational Immunology, 2000, vol. 12, n. 4, p. 547-554es
dc.identifier.issn0953-8178es
dc.identifier.urihttps://uvadoc.uva.es/handle/10324/47500
dc.descriptionProducción Científicaes
dc.description.abstractTHP-1 monocytic/macrophage cells were stimulated via their FcγR receptors with insolubleaggregates of human IgG and the production of the C–C chemokine monocyte chemoattractantprotein (MCP)-1 assayed. A dose- and time-dependent production of MCP-1 comparable to thatproduced by the most potent agonists could be detected in the culture medium by a sensitiveELISA assay. This was accompanied by a parallel activation of the transcription factor NF-κBasjudged from both the appearance ofκB-binding activity containing p50/p65 NF-κB/Rel complexes inthe nuclear extract and the disappearance of the NF-κB inhibitor IκB-αin the cell lysate. Incontrast, IκB-βand IκB-εexpression was not modified, thus pointing to the occurrence of aselective degradation of IκB-αunder those conditions. Attempts to modulate MCP-1 productionwith compounds that display inhibitory effects on the activation of NF-κB such as the proteasomeinhibitorN-acetyl-leucinyl-leucinyl-norleucinal, the antioxidant pyrrolidine dithiocarbamate and thesalicylate derivative 2-hydroxy-4-trifluoromethylbenzoic acid showed a parallel effect on bothMCP-1 production and NF-κB activation, thus pointing to the involvement ofκB-binding sites onthe transcriptional regulation of MCP-1 production. Our findings suggest the existence inmonocytic cells of a signaling mechanism initiated by cross-linking of low-affinity FcγR, most likelyof the FcγRII family since THP-1 cells do not express FcγRIII receptors, that involves activation ofNF-κB associated to the proteolytic degradation of IκB-αand leads to the transcriptional up-regulation of MCP-1es
dc.format.mimetypeapplication/pdfes
dc.language.isoenges
dc.publisherOxford University Presses
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subject.classificationChemokineses
dc.subject.classificationFc receptorses
dc.subject.classificationMonocyteses
dc.subject.classificationMacrophageses
dc.subject.classificationQuimiocinases
dc.subject.classificationMonocitoses
dc.subject.classificationMacrófagoses
dc.titleStimulation of FcγR receptors induces monocyte chemoattractant protein-1 in the human monocytic cell line THP-1 by a mechanism involving IκB-α degradation and formation of p50/p65 NF-κB/Rel complexeses
dc.typeinfo:eu-repo/semantics/articlees
dc.rights.holder© Oxford University Presses
dc.identifier.doi10.1093/intimm/12.4.547es
dc.relation.publisherversionhttps://academic.oup.com/intimm/article/12/4/547/693690es
dc.identifier.publicationfirstpage547es
dc.identifier.publicationissue4es
dc.identifier.publicationlastpage554es
dc.identifier.publicationtitleInternational Immunologyes
dc.identifier.publicationvolume12es
dc.peerreviewedSIes
dc.description.projectThis study has been supported by grants from Plan Nacional de Salud y Farmacia (grants SAF98-0176 and 1FD97-0590)es
dc.identifier.essn1460-2377es
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersiones
dc.subject.unesco24 Ciencias de la Vidaes


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