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dc.contributor.authorAlonso Alonso, María Teresa 
dc.contributor.authorBarrero, María José
dc.contributor.authorMichelena, Pedro
dc.contributor.authorCarnicero Gila, Estela María 
dc.contributor.authorCuchillo, Inmaculada
dc.contributor.authorGarcía, Antonio G.
dc.contributor.authorGarcía-Sancho Martín, Francisco Javier 
dc.contributor.authorMontero Zoccola, María Teresa 
dc.contributor.authorÁlvarez Martín, Javier 
dc.date.accessioned2014-09-15T11:53:11Z
dc.date.available2014-09-15T11:53:11Z
dc.date.issued1999
dc.identifier.citationJournal of Cell Biology, Enero 1999, vol. 144, n. 2, p. 241-254es
dc.identifier.issn0021-9525es
dc.identifier.urihttp://uvadoc.uva.es/handle/10324/5951
dc.descriptionProducción Científicaes
dc.description.abstractThe presence and physiological role of Ca 2+ induced Ca 2+ release (CICR) in nonmuscle excitable cells has been investigated only indirectly through measurements of cytosolic [Ca 2+] ([Ca 2+]c ). Using targeted aequorin, we have directly monitored [Ca 2+] changes inside the ER ([Ca 2+] ER ) in bovine adrenal chromaffin cells. Ca 2+ entry induced by cell depolarization triggered a transient Ca 2+release from the ER that was highly dependent on [Ca 2+] ER and sensitized by low concentrations of caffeine. Caffeine-induced Ca 2+ release was quantal in nature due to modulation by [Ca 2+] ER . Whereas caffeine released essentially all the Ca 2+ from the ER, inositol 1,4,5-trisphosphate (InsP3)- producing agonists released only 60Ð80%. Both InsP3 and caffeine emptied completely the ER in digitoninpermeabilized cells whereas cyclic ADP-ribose had no effect. Ryanodine induced permanent emptying of the Ca 2+ stores in a use-dependent manner after activation by caffeine. Fast confocal [Ca 2+]c measurements showed that the wave of [C 2+]c induced by 100-ms depolarizing pulses in voltage-clamped cells was delayed and reduced in intensity in ryanodine-treated cells. Our results indicate that the ER of chromaffin cells behaves mostly as a single homogeneous thapsigargin-sensitive Ca 2+ pool that can release Ca 2+ both via InsP3 receptors or CICR.es
dc.format.mimetypeapplication/pdfes
dc.language.isoenges
dc.publisherThe Rockefeller University Press,es
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectCalcio - Metabolismoes
dc.titleCa 2+ induced Ca 2+ Release in Chromaffin Cells Seen from inside the ER with Targeted Aequorines
dc.typeinfo:eu-repo/semantics/articlees
dc.identifier.publicationfirstpage241es
dc.identifier.publicationissue2es
dc.identifier.publicationlastpage254es
dc.identifier.publicationtitleJournal of Cell Biologyes
dc.identifier.publicationvolume144es
dc.peerreviewedSIes
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International


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