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dc.contributor.authorStaubli, Sebastian Manuel
dc.contributor.authorCerino, Giulia
dc.contributor.authorGonzález de Torre, Israel 
dc.contributor.authorAlonso Rodrigo, Matilde 
dc.contributor.authorOertli, Daniel
dc.contributor.authorEckstein, Friedrich
dc.contributor.authorGlatz, Katharina
dc.contributor.authorRodríguez Cabello, José Carlos 
dc.contributor.authorMarsano, Anna
dc.date.accessioned2017-07-27T10:48:56Z
dc.date.available2017-07-27T10:48:56Z
dc.date.issued2017
dc.identifier.citationBiomaterials Volume 135, August 2017, Pages 30-41es
dc.identifier.urihttp://uvadoc.uva.es/handle/10324/24758
dc.descriptionProducción Científicaes
dc.description.abstractThe control of the in vivo vascularization of engineered tissue substitutes is essential in order to obtain either a rapid induction or a complete inhibition of the process (e.g. in muscles and hyaline-cartilage, respectively). Among the several polymers available, Elastin-Like Recombinamers (ELR)-based hydrogel stands out as a promising material for tissue engineering thanks to its viscoelastic properties, non-toxicity, and non-immunogenicity. In this study, we hypothesized that varying the cell adhesion properties of ELR-hydrogels could modulate the high angiogenic potential of adipose tissue-derived stromal vascular fraction (SVF) cells, predominantly composed of endothelial/mural and mesenchymal cells. Human SVF cells, embedded in RGD-REDV-bioactivated or unmodified ELR-hydrogels, were implanted in rat subcutaneous pockets either immediately or upon 5-day-culture in perfusion-bioreactors. Perfusion-based culture enhanced the endothelial cell cord-like-organization and the release of pro-angiogenic factors in functionalized constructs. While in vivo vascularization and host cell infiltration within the bioactivated gels were highly enhanced, the two processes were strongly inhibited in non-functionalized SVF-based hydrogels up to 28 days. ELR-based hydrogels showed a great potential to determine the successful integration of engineered substitutes thanks to their capacity to finely control the angiogenic/inflammation process at the recipient site, even in presence of SVF cells.es
dc.format.mimetypeapplication/pdfes
dc.language.isoenges
dc.publisherElsevieres
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.classificationAngiogénesises
dc.titleControl of angiogenesis and host response by modulating the cell adhesion properties of an Elastin-Like Recombinamer-based hydrogeles
dc.typeinfo:eu-repo/semantics/articlees
dc.identifier.doi10.1016/j.biomaterials.2017.04.047es
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0142961217302843es
dc.peerreviewedSIes
dc.description.projectMinisterio de Economía, Industria y Competitividad (Project MAT-2010-15982, MAT2010- 15310, PRI-PIBAR-2011-1403 and MAT2012-38043-C02-01)es
dc.description.projectJunta de Castilla y León (programa de apoyo a proyectos de investigación – Ref.VA152A12-2, VA244U13 and VA155A12-2)es
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/H2020/642687
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP//278557
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/H2020/646075
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/317304
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International


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