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dc.contributor.authorCipriani, Filippo
dc.contributor.authorDominik Bernhagen
dc.contributor.authorGarcía Arévalo, María del Carmen
dc.contributor.authorGonzález de Torre, Israel 
dc.contributor.authorTimmerman, Peter
dc.contributor.authorRodríguez Cabello, José Carlos 
dc.date.accessioned2019-03-04T08:22:35Z
dc.date.issued2019
dc.identifier.citationBiomedical Materials, 2019 (in press)es
dc.identifier.issn1748-6041es
dc.identifier.urihttp://uvadoc.uva.es/handle/10324/34880
dc.descriptionProducción Científicaes
dc.description.abstractBiomaterial design in tissue engineering aims to identify appropriate cellular microenvironments in which cells can grow and guide new tissue formation. Despite the large diversity of synthetic polymers available for regenerative medicine, most of them fail to fully match the functional properties of their native counterparts. In contrast, the few biological alternatives employed as biomaterials lack the versatility that chemical synthesis can offer. Herein, we studied the HUVEC adhesion and proliferation properties of elastin-like recombinamers (ELRs) that were covalently functionalized with each three high-affinity and selectivity αvβ3- and α5β1-binding bicyclic RGD peptides. Next to the bicycles, ELRs were also functionalized with various integrin-binding benchmark peptides, i.e. knottin-RGD, cyclo-[KRGDf] and GRGDS, allowing for better classification of the obtained results. Covalent functionalization with the RGD peptides, as validated by MALDI-TOF analysis, guarantees flexibility and minimal steric hindrance for interactions with cellular integrins. In addition to the covalently modified RGD-ELRs, we also synthesized another benchmark ELR comprising RGD as part of the backbone. HUVEC adhesion and proliferation analysis using the PicoGreen® assay revealed a higher short-term adhesion and proliferative capacity of cells on ELR surfaces functionalized with high affinity, integrin-binding bicyclic RGD-peptides compared with the ELRs containing RGD in the backbone.es
dc.format.mimetypeapplication/pdfes
dc.language.isoenges
dc.publisherIOP Publishinges
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectMedicina regenerativaes
dc.subjectRegenerative medicinees
dc.titleBicyclic RGD peptides with high integrin α<sub>v</sub>β<sub>3</sub> and α<sub>5</sub>β<sub>1</sub> affinity promote cell adhesion on elastin-like recombinamerses
dc.typeinfo:eu-repo/semantics/articlees
dc.identifier.doihttps://doi.org/10.1088/1748-605X/aafd83es
dc.relation.publisherversionhttps://iopscience.iop.org/article/10.1088/1748-605X/aafd83es
dc.peerreviewedSIes
dc.description.embargo2020-01-20es
dc.description.lift2020-01-20
dc.description.projectEuropean Commission (NMP-2014-646075, MSCA-ITN-2014-ETN-642687)es
dc.description.projectMinisterio de Economía, Industria y Competitividad (Projects PCIN-2015-010, 26 MAT2015-68901-R, MAT2016-78903-R)es
dc.description.projectJunta de Castilla y León (programa de apoyo a proyectos de investigación - Ref. VA015U16)es
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International


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