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dc.contributor.authorCosta, André da
dc.contributor.authorPereira, A.
dc.contributor.authorGomes, A
dc.contributor.authorRodríguez Cabello, José Carlos 
dc.contributor.authorCasal, M.
dc.contributor.authorMachado, Raúl
dc.date.accessioned2019-04-04T10:10:11Z
dc.date.issued2018
dc.identifier.citationNew Biotechnology, 2018, vol. 46. p. 45-53es
dc.identifier.issn1871-6784es
dc.identifier.urihttp://uvadoc.uva.es/handle/10324/35332
dc.descriptionProducción Científicaes
dc.description.abstractWith the lack of new chemical antibiotics and the increasing pathogen resistance to those available, new alternatives are being explored. Antimicrobial peptides (AMPs) with a broad range of effects such as antibacterial, antifungal, and antiviral, arise as one of the options. These can be produced by recombinant DNA technology however, the chromatographic methods used for peptide purification are costly and time consuming. Here, we describe the design, production, purification and assessment of the antibacterial activity of the human peptide Hepcidin, using an elastin-like recombinamer as fusion partner. The recombinant protein Hep-A200 was produced in Escherichia coli and purified by a non-chromatographic procedure, exploiting the thermal properties of A200 elastin-like recombinamer. The recombinant polymer Hep-A200 retained antibacterial activity against Gram-positive and Gram-negative species.es
dc.format.mimetypeapplication/pdfes
dc.language.isoenges
dc.publisherElsevieres
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.subject.classificationHepcidina bioactivaes
dc.subject.classificationBioactive hepcidines
dc.subject.classificationPéptido antimicrobianoes
dc.subject.classificationAntimicrobial peptidees
dc.subject.classificationRecombinantes tipo elastinaes
dc.subject.classificationElastin-like recombinameres
dc.subject.classificationActividad antimicrobianaes
dc.subject.classificationAntimicrobial activity.es
dc.titleProduction of bioactive hepcidin by recombinant DNA tagging with an elastin-like recombinameres
dc.typeinfo:eu-repo/semantics/articlees
dc.rights.holder© 2019 Elsevieres
dc.identifier.doihttps://doi.org/10.1016/j.nbt.2018.07.001es
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S1871678418300177es
dc.peerreviewedSIes
dc.description.embargo2019-11-25es
dc.description.lift2019-11-25
dc.description.projectCOMPETE2020. Programa Operacional Competitividade e Internacionalização (POCI strategic program UID/BIA/04050/2013 (POCI-01-0145-FEDER-007569)es
dc.description.projectNorte 2020. Operational Programme of the North Region of Portugal (Project FunBioPlas (grant number ERA-IB-15-089 and FCT reference ERA-IB-2-6/0004/2014)es


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