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dc.contributor.authorAcosta Rodríguez, Sergio 
dc.contributor.authorQuintanilla Sierra, Luis 
dc.contributor.authorAlonso Rodrigo, Matilde 
dc.contributor.authorAparicio, Conrado
dc.contributor.authorRodríguez Cabello, José Carlos 
dc.date.accessioned2020-01-29T08:59:00Z
dc.date.available2020-01-29T08:59:00Z
dc.date.issued2019
dc.identifier.citationACS Biomaterials Science & Engineering, 2019, vol. 5, n. 9. p. 4708-4716es
dc.identifier.issn2373-9878es
dc.identifier.urihttp://uvadoc.uva.es/handle/10324/40376
dc.descriptionProducción Científicaes
dc.description.abstractNosocomial infections are one of the most frequent causes of indwelling biomedical device failure. In this regard, the use of antibiofilm nanocoatings based on antimicrobial peptides (AMPs) is a promising alternative to prevent multiresistant biofilm infections. However, the limitations of chemical production impede the large-scale development of advanced antimicrobial materials that improve the properties of AMPs. Herein, we present a multifunctional modular design for the recombinant coproduction of self-assembled monolayers (SAMs) based on AMPs and elastin-like recombinamers (ELRs), which combine the antimicrobial properties of a designer AMP, GL13K, and low-fouling activity of an ELR in a synergistic manner. The inclusion of a grafting domain intended for oriented tethering onto surfaces allowed the recombinant polymers to be covalently immobilized onto model gold surfaces. The antibiofilm properties against two of the bacterial strains most frequently responsible for indwelling medical device-associated infections, namely Staphylococcus epidermidis and Staphylococcus aureus, were then evaluated. GL13K peptide was found to provide antibiofilm properties to the surface, with these being synergistically enhanced by the antifouling effect of the ELR. This new design offers a promising tool for the development of advanced AMP-based nanocoatings for medical devices with powerful and enhanced features.es
dc.format.mimetypeapplication/pdfes
dc.language.isoenges
dc.publisherACS Publicationses
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subject.classificationAntimicrobial peptideses
dc.subject.classificationPéptido antimicrobianoes
dc.subject.classificationSelf-assembled monolayerses
dc.subject.classificationMonocapas autoensambladases
dc.subject.classificationElastin-like recombinamerses
dc.subject.classificationRecombinantes tipo elastinaes
dc.subject.classificationBacterial strainses
dc.subject.classificationCepas bacterianases
dc.titleRecombinant AMP/Polypeptide Self-Assembled Monolayers with Synergistic Antimicrobial Properties for Bacterial Strains of Medical Relevancees
dc.typeinfo:eu-repo/semantics/articlees
dc.rights.holder© 2019 American Chemical Societyes
dc.identifier.doi10.1021/acsbiomaterials.9b00247es
dc.relation.publisherversionhttps://pubs.acs.org/doi/abs/10.1021/acsbiomaterials.9b00247es
dc.identifier.publicationfirstpage4708es
dc.identifier.publicationissue9es
dc.identifier.publicationlastpage4716es
dc.identifier.publicationtitleACS Biomaterials Science & Engineeringes
dc.identifier.publicationvolume5es
dc.peerreviewedSIes
dc.description.projectComisión Europea (project NMP-2014-646075)es
dc.description.projectMinisterio de Economía, Industria y Competitividad (project PCIN-2015-010 / MAT2015-68901-R / MAT2016-78903-R)es
dc.description.projectJunta de Castilla y León (project VA317P18)es
dc.identifier.essn2373-9878es
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.type.hasVersioninfo:eu-repo/semantics/acceptedVersiones


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