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    • Dpto. Producción Vegetal y Recursos Forestales
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    Por favor, use este identificador para citar o enlazar este ítem:http://uvadoc.uva.es/handle/10324/40820

    Título
    The Fusarium Circinatum gene Fcrho1, encoding a putative Rho1 GTPase, is involved in vegetative growth but dispensable for pathogenic development
    Autor
    Muñoz Adalia, Emigdio JordanAutoridad UVA Orcid
    Cañizares Ruiz, María del Carmen
    Fernández Fernández, María MercedesAutoridad UVA Orcid
    Díez Casero, Julio JavierAutoridad UVA Orcid
    García Pedrajas, María Dolores
    Año del Documento
    2018
    Editorial
    MDPI
    Descripción
    Producción Científica
    Documento Fuente
    Forests, 2018, vol. 9, n. 11. 12 p.
    Resumo
    Fusarium circinatum is the causal agent of pine pitch canker (PPC), one of the most devastating forest diseases worldwide. This fungus causes severe damping-off in pine seedlings and growth reduction, wilting and the development of cankers in pine forests and plantations. A draft of the complete genome sequence of this phytopathogen was recently made available. This information was used to annotate in silico the gene Fcrho1 as a putative homolog of Rho1 GTPase genes. In this study, we generated Fcrho1 deletion mutants in two F. circinatum wildtype strains isolated from damaged trees in northern Spain. For that, we used a modified version of the OSCAR methodology, an approach not previously used in F. circinatum that allows the generation of deletion constructs in a single cloning step. The conidiation and spore germination of the resulting deletion mutants were not affected, neither the hyphal morphology. However, the mutant strains showed significantly reduced growth in vitro and more foamy macroscopic hyphal morphology than their corresponding ectopic and wildtype strains. Finally, an in vivo virulence assay showed that the reduced in vitro growth rate characteristic to the deletion mutants does not impact their pathogenicity.
    Materias Unesco
    3106 Ciencia Forestal
    Palabras Clave
    Cell signalling
    Señalización celular
    Deletion mutants
    Mutantes por delección
    Pine pitch canker disease
    Chancro resinoso del pino
    ISSN
    1999-4907
    Revisión por pares
    SI
    DOI
    10.3390/f9110684
    Patrocinador
    Ministerio de Economía, Industria y Competitividad - Fondo Europeo de Desarrollo Regional (project AGL2015-69370-R)
    Ministerio de Economía, Industria y Competitividad (project AGL2012-39912 / AGL2016-80048-R)
    European Cooperation in Science and Technology (COST Action FP1406 PINESTRENGTH)
    Junta de Castilla y León - Fondo Europeo de Desarrollo Regional (grant ORDEN EDU/1083/2013)
    Version del Editor
    https://www.mdpi.com/1999-4907/9/11/684
    Propietario de los Derechos
    © 2018 MDPI
    Idioma
    eng
    URI
    http://uvadoc.uva.es/handle/10324/40820
    Tipo de versión
    info:eu-repo/semantics/publishedVersion
    Derechos
    openAccess
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    Universidad de Valladolid

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