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dc.contributor.authorSalvo Comino, Coral 
dc.contributor.authorGonzález Gil, Alfonso
dc.contributor.authorRodríguez Valentín, Javier
dc.contributor.authorGarcía Hernández, Celia 
dc.contributor.authorMartín Pedrosa, Fernando 
dc.contributor.authorGarcía Cabezón, Ana Cristina 
dc.contributor.authorRodríguez Méndez, María Luz 
dc.date.accessioned2020-05-28T13:11:03Z
dc.date.available2020-05-28T13:11:03Z
dc.date.issued2020
dc.identifier.citationSensors, 2020, vol. 20, n. 7. 12 p.es
dc.identifier.issn1424-8220es
dc.identifier.urihttp://uvadoc.uva.es/handle/10324/40943
dc.descriptionProducción Científicaes
dc.description.abstractBiosensor platforms consisting of layer by layer films combining materials with different functionalities have been developed and used to obtain improved catechol biosensors. Tyrosinase (Tyr) or laccase (Lac) were deposited onto LbL films formed by layers of a cationic linker (chitosan, CHI) alternating with layers of anionic electrocatalytic materials (sulfonated copper phthalocyanine, CuPcS or gold nanoparticles, AuNP). Films with different layer structures were successfully formed. Characterization of surface roughness and porosity was carried out using AFM. Electrochemical responses towards catechol showed that the LbL composites efficiently improved the electron transfer path between Tyr or Lac and the electrode surface, producing an increase in the intensity over the response in the absence of the LbL platform. LbL structures with higher roughness and pore size facilitated the diffusion of catechol, resulting in lower LODs. The [(CHI)-(AuNP)-(CHI)-(CuPcS)]2-Tyr showed an LOD of 8.55∙10−4 μM, which was one order of magnitude lower than the 9.55·10−3 µM obtained with [(CHI)-(CuPcS)-(CHI)-(AuNP)]2-Tyr, and two orders of magnitude lower than the obtained with other nanostructured platforms. It can be concluded that the combination of adequate materials with complementary activity and the control of the structure of the platform is an excellent strategy to obtain biosensors with improved performances.es
dc.format.mimetypeapplication/pdfes
dc.language.isoenges
dc.publisherMDPIes
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subject.classificationCatecholes
dc.subject.classificationCatecoles
dc.subject.classificationGold nanoparticleses
dc.subject.classificationNanopartículas de oroes
dc.subject.classificationLaccaseses
dc.subject.classificationLacasases
dc.subject.classificationTyrosinasees
dc.subject.classificationTirosinasaes
dc.titleBiosensors Platform Based on Chitosan/AuNPs/Phthalocyanine Composite Films for the Electrochemical Detection of Catechol. The Role of the Surface Structurees
dc.typeinfo:eu-repo/semantics/articlees
dc.rights.holder© 2020 MDPIes
dc.identifier.doi10.3390/s20072152es
dc.relation.publisherversionhttps://www.mdpi.com/1424-8220/20/7/2152es
dc.peerreviewedSIes
dc.description.projectMinisterio de Ciencia, Innovación y Universidades - Fondo Europeo de Desarrollo Regional (project RTI2018-097990-B-100)es
dc.description.projectJunta de Castilla y Leon - Fondo Europeo de Desarrollo Regional (project VA275P18)es
dc.description.projectInfraestructuras Red de Castilla y León (grant UVA01)es
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersiones
dc.subject.unesco2307 Química Físicaes
dc.subject.unesco2303 Química Inorgánicaes


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