• español
  • English
  • français
  • Deutsch
  • português (Brasil)
  • italiano
    • español
    • English
    • français
    • Deutsch
    • português (Brasil)
    • italiano
    • español
    • English
    • français
    • Deutsch
    • português (Brasil)
    • italiano
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Navegar

    Todo o repositórioComunidadesPor data do documentoAutoresAssuntosTítulos

    Minha conta

    Entrar

    Estatística

    Ver as estatísticas de uso

    Compartir

    Ver item 
    •   Página inicial
    • PRODUÇÃO CIENTÍFICA
    • Departamentos
    • Dpto. Bioquímica y Biología Molecular y Fisiología
    • DEP06 - Artículos de revista
    • Ver item
    •   Página inicial
    • PRODUÇÃO CIENTÍFICA
    • Departamentos
    • Dpto. Bioquímica y Biología Molecular y Fisiología
    • DEP06 - Artículos de revista
    • Ver item
    • español
    • English
    • français
    • Deutsch
    • português (Brasil)
    • italiano

    Exportar

    RISMendeleyRefworksZotero
    • edm
    • marc
    • xoai
    • qdc
    • ore
    • ese
    • dim
    • uketd_dc
    • oai_dc
    • etdms
    • rdf
    • mods
    • mets
    • didl
    • premis

    Citas

    Por favor, use este identificador para citar o enlazar este ítem:http://uvadoc.uva.es/handle/10324/41034

    Título
    Effect of mutation of cytoplasmic receptor domain and of genistein on transport of acidic fibroblast growth factor into cells
    Autor
    Muñoz Martínez, RaquelAutoridad UVA Orcid
    Klingenberg, Olav
    Wiedlocha, Antoni
    Rapak, Andrzej
    Falnes, Pål
    Olsnes, Sjur
    Año del Documento
    1997
    Editorial
    Springer Nature
    Descripción
    Producción Científica
    Documento Fuente
    Oncogene, 1997, vol. 15, n. 5. p. 525-536
    Resumo
    Acidic fibroblast growth factor (aFGF) binds to specific transmembrane receptors and is partly transported to a nuclear location. To study this transport we made a kinase-negative mutant of FGF receptor 4 as well as one where the major part of the cytoplasmic receptor domain was deleted, and expressed them in U2OSDr1 cells that lack functional FGF receptors. All receptors mediated endocytic uptake of aFGF. Translocation of the growth factor across cellular membranes was assayed using aFGF with a C-terminal CAAX-motif, which signals addition of a farnesyl group onto the protein once in the cytosol. CAAX-tagged aFGF was farnesylated when incubated with cells containing wild-type or kinase-negative receptors. It was not farnesylated in cells expressing the deleted receptor, or when the incubation was in the presence of genistein. aFGF incubated with cells transfected with wild-type or kinase-negative receptors, but not with the deleted receptor, was partly recovered from the nuclear fraction in the absence, but not in the presence of genistein. The data indicate that the cytoplasmic receptor domain, but not the active kinase, is required for transport of the growth factor into cells, and that genistein inhibits the process.
    Palabras Clave
    Receptor mutant
    Receptor mutante
    Genistein
    Genisteína
    Farnesylation
    Farnesilación
    ISSN
    1476-5594
    Revisión por pares
    SI
    DOI
    10.1038/sj.onc.1201226
    Patrocinador
    Ministerio de Educación y Formación Profesional - Unión Europea (grant ERB4001GT954487)
    Version del Editor
    https://www.nature.com/articles/1201226
    Propietario de los Derechos
    © 1997 Springer Nature
    Idioma
    eng
    URI
    http://uvadoc.uva.es/handle/10324/41034
    Tipo de versión
    info:eu-repo/semantics/publishedVersion
    Derechos
    openAccess
    Aparece en las colecciones
    • DEP06 - Artículos de revista [352]
    Mostrar registro completo
    Arquivos deste item
    Nombre:
    Effect-of-mutation-of-cytoplasmic-receptor.pdf
    Tamaño:
    621.6Kb
    Formato:
    Adobe PDF
    Thumbnail
    Visualizar/Abrir

    Universidad de Valladolid

    Powered by MIT's. DSpace software, Version 5.10