Inmovilización de aminoacilasas de streptomyces ambofaciens

Abstract

Enzymes are widely used as biocatalysts for different reactions. When operating with free-form enzymes they are in a liquid state so it will be difficult to separate them from the reaction product if complex methods are not used. If the enzyme is not fully recovered, the product will be contaminated. Besides, by using the enzyme in this way, it cannot be reused in other reactions and the stability time of the enzyme for the operation is low. These problems could be solved by immobilizing the enzymes since they are more stables when fixed to a carrier and since they are in a solid form they are better handled, easier to separate from the product and can be reused. Despite the advantages that the immobilization can have, when fixing the enzymes to a carrier they can lose activity. That is why is necessary to choose the support correctly and the immobilization method for the fixation to be effective. In the laboratory aminoacylases are produced from Streptomyces ambofaciens; however, it is necessary to immobilize them because as it is been said, in their free form they are very unstable. The objectives of the present work is to be able to compare the immobilizations of these enzymes, the aminoacylases, in different supports, silicon or carbonated. In addition, optimal conditions have to be found to carry out the immobilization step, both in terms of temperature and pH, the buffer used and the support in which the immobilization is carried out

Las enzimas son muy utilizadas como biocatalizadores en diferentes reacciones. Cuando se opera con enzimas en su forma libre están en estado líquido por lo que son difíciles de manejar y su estabilidad dura poco tiempo. Estos problemas se solucionan inmovilizando las enzimas en un soporte.