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    Por favor, use este identificador para citar o enlazar este ítem:http://uvadoc.uva.es/handle/10324/44597

    Título
    Cell cycle-dependent expression of Kv3.4 channels modulates proliferation of human uterine artery smooth muscle cells
    Autor
    Miguel Velado, Eduardo
    Pérez Carretero, Francisco D.
    Colinas, Olaia
    Cidad Velasco, María Del PilarAutoridad UVA Orcid
    Heras i Fortuny, Maria Magdalena
    López López, José RamónAutoridad UVA Orcid
    Pérez García, María TeresaAutoridad UVA Orcid
    Año del Documento
    2010
    Editorial
    Oxford University Press
    Descripción
    Producción Científica
    Documento Fuente
    Cardiovascular Research, 2010, vol. 86, n. 3. p. 383-391
    Zusammenfassung
    Aims: Vascular smooth muscle cell (VSMC) proliferation is involved in cardiovascular pathologies associated with unwanted arterial wall remodelling. Coordinated changes in the expression of several K+ channels have been found to be important elements in the phenotypic switch of VSMCs towards proliferation. We have previously demonstrated the association of functional expression of Kv3.4 channels with proliferation of human uterine VSMCs. Here, we sought to gain deeper insight on the relationship between Kv3.4 channels and cell cycle progression in this preparation. Methods and results: Expression and function of Kv3.4 channels along the cell cycle was explored in uterine VSMCs synchronized at different checkpoints, combining real-time PCR, western blotting, and electrophysiological techniques. Flow cytometry, Ki67 expression and BrdU incorporation techniques allowed us to explore the effects of Kv3.4 channels blockade on cell cycle distribution. We found cyclic changes in Kv3.4 and MiRP2 mRNA and protein expression along the cell cycle. Functional studies showed that Kv3.4 current amplitude and Kv3.4 channels contribution to cell excitability increased in proliferating cells. Finally, both Kv3.4 blockers and Kv3.4 knockdown with siRNA reduced the proportion of proliferating VSMCs. Conclusion: Our data indicate that Kv3.4 channels exert a permissive role in the cell cycle progression of proliferating uterine VSMCs, as their blockade induces cell cycle arrest after G2/M phase completion. The modulation of resting membrane potential (VM) by Kv3.4 channels in proliferating VSMCs suggests that their role in cell cycle progression could be at least in part mediated by their contribution to the hyperpolarizing signal needed to progress through the G1 phase.
    Materias Unesco
    32 Ciencias Médicas
    Palabras Clave
    Vascular smooth muscle
    Músculo liso vascular
    Potassium channels
    Canales de potasio
    Cell proliferation
    Proliferación celular
    Electrophysiology
    Electrofisiología
    ISSN
    1755-3245
    Revisión por pares
    SI
    DOI
    10.1093/cvr/cvq011
    Patrocinador
    Ministerio de Sanidad, Consumo y Bienestar Social - Instituto de Salud Carlos III (grants R006/009 and PI041044)
    Ministerio de Ciencia, Innovación y Universidades (grants BFU2004-05551 and BFU2007-61524)
    Junta de Castilla y León (grant GR242)
    Version del Editor
    https://academic.oup.com/cardiovascres/article/86/3/383/317304
    Propietario de los Derechos
    © 2010 Oxford University Press
    Idioma
    eng
    URI
    http://uvadoc.uva.es/handle/10324/44597
    Tipo de versión
    info:eu-repo/semantics/publishedVersion
    Derechos
    openAccess
    Aparece en las colecciones
    • DEP06 - Artículos de revista [353]
    • IBGM - Artículos de revista [79]
    • VASCUMIT - Artículos de revista [47]
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    Nombre:
    Cell-cycle-dependent-expression.pdf
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    Universidad de Valladolid

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