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dc.contributor.authorChamero, Pablo
dc.contributor.authorVillalobos Jorge, Carlos
dc.contributor.authorAlonso Alonso, María Teresa 
dc.contributor.authorGarcía-Sancho Martín, Francisco Javier 
dc.date.accessioned2021-01-15T10:42:29Z
dc.date.available2021-01-15T10:42:29Z
dc.date.issued2002
dc.identifier.citationJournal of Biological Chemistry, 2002, vol. 277, n. 52. p. 50226-50229es
dc.identifier.issn0021-9258es
dc.identifier.urihttp://uvadoc.uva.es/handle/10324/45020
dc.descriptionProducción Científicaes
dc.description.abstractCa2+ signals may regulate gene expression. The increase of the cytosolic Ca2+concentration ([Ca2+]c) promotes activation and/or nuclear import of some transcription factors, but others require the increase of the nuclear Ca2+ concentration ([Ca2+]N) for activation. Whether the nuclear envelope may act as a diffusion barrier for propagation of [Ca2+]c signals remains controversial. We have studied the spreading of Ca2+ from the cytosol to the nucleus by comparing the cytosolic and the nuclear Ca2+signals reported by targeted aequorins in adrenal chromaffin, PC12, and GH3 pituitary cells. Strong stimulation of either Ca2+ entry (by depolarization with high K+ or acethylcholine) or Ca2+ release from the intracellular Ca2+ stores (by stimulation with caffeine, UTP, bradykinin, or thyrotropin-releasing hormone (TRH)) produced similar Ca2+ signals in cytosol and nucleus. In contrast, both spontaneous and TRH-stimulated oscillations of cytosolic Ca2+ in single GH3 cells were considerably dampened during propagation to the nucleus. These results are consistent with the existence of a kinetic barrier that filters high frequency physiological [Ca2+]c oscillations without disturbing sustained [Ca2+]cincreases. Thus, encoding of the Ca2+ signal may allow differential control of Ca2+-dependent mechanisms located at either the cytosol or the nucleus.es
dc.format.mimetypeapplication/pdfes
dc.language.isoenges
dc.publisherElsevieres
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.subject.classificationCytosoles
dc.subject.classificationCitosoles
dc.subject.classificationCalcium signalinges
dc.subject.classificationSeñalización de calcioes
dc.subject.classificationCell nucleuses
dc.subject.classificationNúcleo celulares
dc.subject.classificationGene expressiones
dc.subject.classificationExpresión génicaes
dc.titleDampening of cytosolic Ca2+ oscillations on propagation to nucleuses
dc.typeinfo:eu-repo/semantics/articlees
dc.rights.holder© 2002 Elsevieres
dc.identifier.doi10.1074/jbc.C200522200es
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0021925819313250es
dc.peerreviewedSIes
dc.description.projectMinisterio de Ciencia, Innovación y Universidades (grant BFI2001-2073)es
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersiones


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