dc.contributor.author | Chamero, Pablo | |
dc.contributor.author | Villalobos Jorge, Carlos | |
dc.contributor.author | Alonso Alonso, María Teresa | |
dc.contributor.author | García-Sancho Martín, Francisco Javier | |
dc.date.accessioned | 2021-01-15T10:42:29Z | |
dc.date.available | 2021-01-15T10:42:29Z | |
dc.date.issued | 2002 | |
dc.identifier.citation | Journal of Biological Chemistry, 2002, vol. 277, n. 52. p. 50226-50229 | es |
dc.identifier.issn | 0021-9258 | es |
dc.identifier.uri | http://uvadoc.uva.es/handle/10324/45020 | |
dc.description | Producción Científica | es |
dc.description.abstract | Ca2+ signals may regulate gene expression. The increase of the cytosolic Ca2+concentration ([Ca2+]c) promotes activation and/or nuclear import of some transcription factors, but others require the increase of the nuclear Ca2+ concentration ([Ca2+]N) for activation. Whether the nuclear envelope may act as a diffusion barrier for propagation of [Ca2+]c signals remains controversial. We have studied the spreading of Ca2+ from the cytosol to the nucleus by comparing the cytosolic and the nuclear Ca2+signals reported by targeted aequorins in adrenal chromaffin, PC12, and GH3 pituitary cells. Strong stimulation of either Ca2+ entry (by depolarization with high K+ or acethylcholine) or Ca2+ release from the intracellular Ca2+ stores (by stimulation with caffeine, UTP, bradykinin, or thyrotropin-releasing hormone (TRH)) produced similar Ca2+ signals in cytosol and nucleus. In contrast, both spontaneous and TRH-stimulated oscillations of cytosolic Ca2+ in single GH3 cells were considerably dampened during propagation to the nucleus. These results are consistent with the existence of a kinetic barrier that filters high frequency physiological [Ca2+]c oscillations without disturbing sustained [Ca2+]cincreases. Thus, encoding of the Ca2+ signal may allow differential control of Ca2+-dependent mechanisms located at either the cytosol or the nucleus. | es |
dc.format.mimetype | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | Elsevier | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.subject.classification | Cytosol | es |
dc.subject.classification | Citosol | es |
dc.subject.classification | Calcium signaling | es |
dc.subject.classification | Señalización de calcio | es |
dc.subject.classification | Cell nucleus | es |
dc.subject.classification | Núcleo celular | es |
dc.subject.classification | Gene expression | es |
dc.subject.classification | Expresión génica | es |
dc.title | Dampening of cytosolic Ca2+ oscillations on propagation to nucleus | es |
dc.type | info:eu-repo/semantics/article | es |
dc.rights.holder | © 2002 Elsevier | es |
dc.identifier.doi | 10.1074/jbc.C200522200 | es |
dc.relation.publisherversion | https://www.sciencedirect.com/science/article/pii/S0021925819313250 | es |
dc.peerreviewed | SI | es |
dc.description.project | Ministerio de Ciencia, Innovación y Universidades (grant BFI2001-2073) | es |
dc.type.hasVersion | info:eu-repo/semantics/publishedVersion | es |