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    Por favor, use este identificador para citar o enlazar este ítem:http://uvadoc.uva.es/handle/10324/45079

    Título
    Dynamics of stimulus-expression coupling as revealed by monitoring of prolactin promoter-driven reporter activity in individual, living mammotropes
    Autor
    Villalobos Jorge, Carlos
    Faught, William J.
    Frawley, L. Stephen
    Año del Documento
    1999
    Editorial
    Oxford University Press
    Descripción
    Producción Científica
    Documento Fuente
    Molecular Endocrinology, 1999, vol. 13, n. 10. p. 1718-1727
    Resumen
    Single-cell paradigms have greatly expanded our knowledge about stimulus-secretion coupling, but the understanding of stimulus-gene expression coupling has lagged behind for lack of a dynamic model sufficiently sensitive to provide single-cell resolution. In the present study, we made continuous indirect measurements within individual, living cells of expression dynamics both before and after treatment with a gene-activating secretagogue. This was accomplished by transfecting (via microinjection) individual, primary mammotropes with a PRL promoter-driven luciferase reporter plasmid, and then quantifying the rate of photonic emissions (reflective of endogenous gene activity). We found that individual cells exhibit spontaneous, random, short-term fluctuations of basal reporter activity and are extremely heterogeneous in terms of responses to a stimulatory agent (TRH). In addition, we found that responses are affected by several factors including the secretory status of the pituitary donor, the manner in which the stimulus is presented, and by the initial level of reporter activity. Moreover, the responsiveness of an individual cell can fluctuate dramatically over time. These results invite speculation that a given cell can “sense” its gene activation state and regulate its response accordingly to satisfy requirements for the corresponding secretory product.
    ISSN
    1944-9917
    Revisión por pares
    SI
    DOI
    10.1210/mend.13.10.0358
    Patrocinador
    National Institutes of Health (grant DK-38215)
    Version del Editor
    https://academic.oup.com/mend/article/13/10/1718/2747795
    Propietario de los Derechos
    © 1999 Oxford University Press
    Idioma
    eng
    URI
    http://uvadoc.uva.es/handle/10324/45079
    Tipo de versión
    info:eu-repo/semantics/publishedVersion
    Derechos
    openAccess
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    • CFC - Artículos de Revista [38]
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    Attribution-NonCommercial-NoDerivs 3.0 UnportedLa licencia del ítem se describe como Attribution-NonCommercial-NoDerivs 3.0 Unported

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