dc.contributor.author | Blanco Rodríguez, Josefa | |
dc.contributor.author | Martínez García, María del Carmen | |
dc.contributor.author | Porras Gallo, María Almudena | |
dc.date.accessioned | 2021-01-22T13:19:34Z | |
dc.date.available | 2021-01-22T13:19:34Z | |
dc.date.issued | 2003 | |
dc.identifier.citation | Reproduction, 2003, vol. 126, n. 5. p. 661-668 | es |
dc.identifier.issn | 1741-7899 | es |
dc.identifier.uri | http://uvadoc.uva.es/handle/10324/45116 | |
dc.description | Producción Científica | es |
dc.description.abstract | In the seminiferous epithelium, both DNA synthesis and
apoptosis occur at equivalent stages in various species, with
apoptosis taking place mainly at the same stages as DNA
replication in the second, third and fourth spermatogonial
generations. As preservation of the cellular associations
found at these stages may have some functional significance, it is important to determine whether there is a
correlation between these cellular events. In this study,
pairs of immunoperoxidase-stained adjacent testis sections
from rats, mice, rabbits and cats in which either bromodeoxyuridine incorporated into the newly synthesized
DNA strand (BrdU labelling) or DNA 3 end labelling
of the apoptotic DNA fragments (TUNEL assay) were
detected were compared. In addition, both events were
analysed in double-labelled sections. These two methods
revealed a clear correlation between the occurrence of
DNA replication in the second to fourth generations of
spermatogonia and most physiological apoptosis taking
place in both spermatogonia and spermatocytes in the
three different mammalian orders (Rodentia, Lagomorpha
and Carnivora). This correlation may result from the
synchronization of mitotic spermatogonial and meiotic
spermatocyte cell cycle checkpoints operating at these
stages. | es |
dc.format.mimetype | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | Bioscientifica | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/ | * |
dc.subject.classification | DNA synthesis | es |
dc.subject.classification | Síntesis de ADN | es |
dc.subject.classification | Spermatogonia | es |
dc.subject.classification | Espermatogonia | es |
dc.subject.classification | Apoptosis | es |
dc.subject.classification | Apoptosis | es |
dc.subject.classification | Spermatocytes | es |
dc.subject.classification | Espermatocitos | es |
dc.title | Correlation between DNA synthesis in the second, third and fourth generations of spermatogonia and the occurrence of apoptosis in both spermatogonia and spermatocytes | es |
dc.type | info:eu-repo/semantics/article | es |
dc.rights.holder | © 2003 Bioscientifica | es |
dc.identifier.doi | 10.1530/rep.0.1260661 | es |
dc.relation.publisherversion | https://rep.bioscientifica.com/view/journals/rep/126/5/661.xml | es |
dc.peerreviewed | SI | es |
dc.description.project | Ministerio de Sanidad, Consumo y Bienestar Social (grant FIS 02/1886) | es |
dc.description.project | Junta de Castilla y León (grant VA 115/01) | es |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Unported | * |
dc.type.hasVersion | info:eu-repo/semantics/publishedVersion | es |