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Título
Elucidating the key environmental parameters during the production of ectoines from biogas by mixed methanotrophic consortia
Autor
Año del Documento
2021
Editorial
Elsevier
Descripción
Producción Científica
Documento Fuente
Journal of Environmental Management, 2021, vol. 298, 113462
Resumo
Anaerobic digestion (AD) is a robust biotechnology for the valorisation of organic waste into biogas. However, the rapid decrease in renewable electricity prices requires alternative uses of biogas. In this context, the engineering of innovative platforms for the bio-production of chemicals from CH4 has recently emerged. The extremolyte and osmoprotectant ectoine, with a market price of ~1000€/Kg, is the industrial flagship of CH4-based bio-chemicals. This work aimed at optimizing the accumulation of ectoines using mixed microbial consortia enriched from saline environments (a salt lagoon and a salt river) and activated sludge, and biogas as feedstock. The influence of NaCl (0, 3, 6, 9 and 12 %) and Na2WO4 (0, 35 and 70 μg L−1) concentrations and incubation temperature (15, 25 and 35 °C) on the stoichiometry and kinetics of the methanotrophic consortia was investigated. Consortia enriched from activated sludge at 15 °C accumulated the highest yields of ectoine and hydroxyectoine at 6 % NaCl (105.0 ± 27.2 and 24.2 ± 5.4 mgextremolyte gbiomass−1, respectively). The consortia enriched from the salt lagoon accumulated the highest yield of ectoine and hydroxyectoine at 9 % NaCl (56.6 ± 2.5 and 51.0 ± 2.0 mgextremolyte gbiomass−1, respectively) at 25 °C. The supplementation of tungsten to the cultivation medium did not impact on the accumulation of ectoines in any of the consortia. A molecular characterization of the enrichments revealed a relative abundance of ectoine-accumulating methanotrophs of 7–16 %, with Methylomicrobium buryatense and Methylomicrobium japanense as the main players in the bioconversion of methane into ectoine.
Palabras Clave
Biogas
Biogás
Ectoine
Ectoína
Methanotrophs
Metanotrofo
ISSN
0301-4797
Revisión por pares
SI
Patrocinador
European Union's Horizon 2020 research and innovation program (grant 837998)
Junta de Castilla y León - Fondo Europeo de Desarrollo Regional (grants CLU 2017–09 and UIC 071)
Junta de Castilla y León - Fondo Europeo de Desarrollo Regional (grants CLU 2017–09 and UIC 071)
Patrocinador
info:eu-repo/grantAgreement/EC/H2020/837998
Propietario de los Derechos
© 2021 The Authors
Idioma
eng
Tipo de versión
info:eu-repo/semantics/publishedVersion
Derechos
openAccess
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