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dc.contributor.authorStojic, Marija
dc.contributor.authorRódenas Rochina, Joaquín
dc.contributor.authorLópez Donaire, María Luisa
dc.contributor.authorGonzález de Torre, Israel 
dc.contributor.authorGonzález Pérez, Miguel 
dc.contributor.authorRodríguez Cabello, José Carlos 
dc.contributor.authorVojtová, Lucy
dc.contributor.authorJorcano Noval, José Luis
dc.contributor.authorVelasco Bayón, Diego
dc.date.accessioned2022-07-13T11:32:06Z
dc.date.available2022-07-13T11:32:06Z
dc.date.issued2021
dc.identifier.citationPolymers, 2021, vol. 13, n. 13, p. 2114es
dc.identifier.issn2073-4360es
dc.identifier.urihttps://uvadoc.uva.es/handle/10324/53937
dc.descriptionProducción Científicaes
dc.description.abstractDermo-epidermal equivalents based on plasma-derived fibrin hydrogels have been ex tensively studied for skin engineering. However, they showed rapid degradation and contraction over time and low mechanical properties which limit their reproducibility and lifespan. In order to achieve better mechanical properties, elasticity and biological properties, we incorporated a elastin-like recombinamer (ELR) network, based on two types of ELR, one modified with azide (SKS-N3 ) and other with cyclooctyne (SKS-Cyclo) chemical groups at molar ratio 1:1 at three different SKS (serine-lysine-serine sequence) concentrations (1, 3, and 5 wt.%), into plasma-derived fibrin hydrogels. Our results showed a decrease in gelation time and contraction, both in the absence and presence of the encapsulated human primary fibroblasts (hFBs), higher mechanical properties and increase in elasticity when SKSs content is equal or higher than 3%. However, hFBs proliferation showed an improvement when the lowest SKS content (1 wt.%) was used but started decreasing when increasing SKS concentration at day 14 with respect to the plasma control. Proliferation of human primary keratinocytes (hKCs) seeded on top of the hybrid-plasma hydrogels containing 1 and 3% of SKS showed no differences to plasma control and an increase in hKCs proliferation was observed for hybrid-plasma hydrogels containing 5 wt.% of SKS. These promising results showed the need to achieve a balance between the reduced contraction, the better mechanical properties and biological properties and indicate the potential of using this type of hydrogel as a testing platform for pharmaceutical products and cosmetics, and future work will elucidate their potential.es
dc.format.mimetypeapplication/pdfes
dc.language.isoenges
dc.publisherMDPIes
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subject.classificationElastines
dc.subject.classificationElastin like recombinamerses
dc.subject.classificationFibrin hydrogelses
dc.subject.classificationElastin-plasma hybrid hydrogelses
dc.subject.classificationSkin tissue engineeringes
dc.subject.classificationBioengineered skines
dc.titleElastin-plasma hybrid hydrogels for skin tissue engineeringes
dc.typeinfo:eu-repo/semantics/articlees
dc.rights.holder© 2021 The Author(s)es
dc.identifier.doi10.3390/polym13132114es
dc.relation.publisherversionhttps://www.mdpi.com/2073-4360/13/13/2114es
dc.identifier.publicationfirstpage2114es
dc.identifier.publicationissue13es
dc.identifier.publicationtitlePolymerses
dc.identifier.publicationvolume13es
dc.peerreviewedSIes
dc.description.projectGobierno Español, (Grant/Award Numbers: FPU15-00448, PID2019-110709RB-100, RED2018-102417-T)es
dc.description.projectJunta de Castilla y León (VA317P18, Infrared2018-UVA06),es
dc.description.projectInterreg. V España-Portugal POCTEP (0624_2IQBIONEURO_6_E)es
dc.description.projectPrograma de Actividades de I + D entre Grupos de Investigación de la Comunidad de Madrid (S2018/BAA-4480)es
dc.description.projectPrograma Estatal de I + D + i Orientada a los Retos de la So-ciedad (RTI2018-101627-B-I00)es
dc.identifier.essn2073-4360es
dc.rightsAtribución 4.0 Internacional*
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersiones
dc.subject.unesco22 Físicaes
dc.subject.unesco23 Químicaes
dc.subject.unesco24 Ciencias de la Vidaes


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