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    Por favor, use este identificador para citar o enlazar este ítem:http://uvadoc.uva.es/handle/10324/5985

    Título
    Mitochondrial free [Ca2+] levels and the permeability transition
    Autor
    Vay, Laura
    Hernández San Miguel, Esther
    Domínguez Lobatón, María CarmenAutoridad UVA Orcid
    Moreno Díaz-Calderón, AlfredoAutoridad UVA Orcid
    Montero Zoccola, María TeresaAutoridad UVA Orcid
    Álvarez Martín, JavierAutoridad UVA Orcid
    Año del Documento
    2009
    Editorial
    Elsevier Ltd.
    Descripción
    Producción Científica
    Documento Fuente
    Cell Calcium, 2009, vol. 45, p. 243-250
    Zusammenfassung
    Mitochondrial Ca2+ activates many processes, from mitochondrial metabolism to opening of the permeability transition pore (PTP) and apoptosis. However, there is considerable controversy regarding the free mitochondrial [Ca2+] ([Ca2+]M) levels that can be attained during cell activation or even in mitochondrial preparations. Studies using fluorescent dyes (rhod-2 or similar), have reported that phosphate precipitation precludes [Ca2+]M from increasing above 2–3 M. Instead, using low-Ca2+-affinity aequorin probes, we have measured [Ca2+]M values more than two orders of magnitude higher. We confirm here these values by making a direct in situ calibration of mitochondrial aequorin, and we show that a prolonged increase in [Ca2+]M to levels of 0.5–1mM was actually observed at any phosphate concentration (0–10mM) during continuous perfusion of 3.5–100 MCa2+-buffers. In spite of this high and maintained (>10 min) [Ca2+]M, mitochondria retained functionality and the [Ca2+]M drop induced by a protonophore was fully reversible. In addition, this high [Ca2+]M did not induce PTP opening unless additional activators (phenyl arsine oxide, PAO) were present. PAO induced a rapid, concentration-dependent and irreversible drop in [Ca2+]M. In conclusion [Ca2+]M levels of 0.5–1mM can be reached and maintained for prolonged periods (>10 min) in phosphate-containing medium, and massive opening of PTP requires additional pore activators.
    Materias (normalizadas)
    Calcio - Metabolismo
    ISSN
    0143-4160
    Revisión por pares
    SI
    DOI
    10.1016/j.ceca.2008.10.007
    Idioma
    eng
    URI
    http://uvadoc.uva.es/handle/10324/5985
    Derechos
    openAccess
    Aparece en las colecciones
    • DEP05 - Artículos de revista [198]
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    Dateien zu dieser Ressource
    Nombre:
    ALVAREZ 23- Mitochondrial free [Ca2+] levels.pdf
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