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dc.contributor.author | Vay, Laura | |
dc.contributor.author | Hernández San Miguel, Esther | |
dc.contributor.author | Domínguez Lobatón, María Carmen | |
dc.contributor.author | Moreno Díaz-Calderón, Alfredo | |
dc.contributor.author | Montero Zoccola, María Teresa | |
dc.contributor.author | Álvarez Martín, Javier | |
dc.date.accessioned | 2014-09-16T11:07:28Z | |
dc.date.available | 2014-09-16T11:07:28Z | |
dc.date.issued | 2009 | |
dc.identifier.citation | Cell Calcium, 2009, vol. 45, p. 243-250 | es |
dc.identifier.issn | 0143-4160 | es |
dc.identifier.uri | http://uvadoc.uva.es/handle/10324/5985 | |
dc.description | Producción Científica | es |
dc.description.abstract | Mitochondrial Ca2+ activates many processes, from mitochondrial metabolism to opening of the permeability transition pore (PTP) and apoptosis. However, there is considerable controversy regarding the free mitochondrial [Ca2+] ([Ca2+]M) levels that can be attained during cell activation or even in mitochondrial preparations. Studies using fluorescent dyes (rhod-2 or similar), have reported that phosphate precipitation precludes [Ca2+]M from increasing above 2–3 M. Instead, using low-Ca2+-affinity aequorin probes, we have measured [Ca2+]M values more than two orders of magnitude higher. We confirm here these values by making a direct in situ calibration of mitochondrial aequorin, and we show that a prolonged increase in [Ca2+]M to levels of 0.5–1mM was actually observed at any phosphate concentration (0–10mM) during continuous perfusion of 3.5–100 MCa2+-buffers. In spite of this high and maintained (>10 min) [Ca2+]M, mitochondria retained functionality and the [Ca2+]M drop induced by a protonophore was fully reversible. In addition, this high [Ca2+]M did not induce PTP opening unless additional activators (phenyl arsine oxide, PAO) were present. PAO induced a rapid, concentration-dependent and irreversible drop in [Ca2+]M. In conclusion [Ca2+]M levels of 0.5–1mM can be reached and maintained for prolonged periods (>10 min) in phosphate-containing medium, and massive opening of PTP requires additional pore activators. | es |
dc.format.mimetype | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | Elsevier Ltd. | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.subject | Calcio - Metabolismo | es |
dc.title | Mitochondrial free [Ca2+] levels and the permeability transition | es |
dc.type | info:eu-repo/semantics/article | es |
dc.identifier.doi | 10.1016/j.ceca.2008.10.007 | es |
dc.identifier.publicationfirstpage | 243 | es |
dc.identifier.publicationlastpage | 250 | es |
dc.identifier.publicationtitle | Cell Calcium | es |
dc.identifier.publicationvolume | 45 | es |
dc.peerreviewed | SI | es |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 International |
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