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Título
Isolation, characterization and biological action of type-1 ribosome-inactivating proteins from tissues of Salsola soda L.
Autor
Año del Documento
2022
Editorial
MDPI
Descripción
Producción Científica
Documento Fuente
Toxins, 2022, Vol. 14, Nº. 8, 566
Abstract
Ribosome-inactivating proteins (RIPs) are known as RNA N-glycosylases. They depurinate the major rRNA, damaging ribosomes and inhibiting protein synthesis. Here, new single-chain (type-1) RIPs named sodins were isolated from the seeds (five proteins), edible leaves (one protein) and roots (one protein) of Salsola soda L. Sodins are able to release Endo’s fragment when incubated with rabbit and yeast ribosomes and inhibit protein synthesis in cell-free systems (IC50 = 4.83–79.31 pM). In addition, sodin 5, the major form isolated from seeds, as well as sodin eL and sodin R, isolated from edible leaves and roots, respectively, display polynucleotide:adenosine glycosylase activity and are cytotoxic towards the Hela and COLO 320 cell lines (IC50 = 0.41–1200 nM), inducing apoptosis. The further characterization of sodin 5 reveals that this enzyme shows a secondary structure similar to other type-1 RIPs and a higher melting temperature (Tm = 76.03 ± 0.30 °C) and is non-glycosylated, as other sodins are. Finally, we proved that sodin 5 possesses antifungal activity against Penicillium digitatum.
Materias (normalizadas)
Toxicology
Proteins - Synthesis
Ribosomes - Structure
Proteínas - Síntesis
Molecular biology
Plant Sciences
Plantas comestibles
Biochemistry
Proteins - Purification
RNA
ARN
Antifungal agents
Materias Unesco
2302.27 Proteínas
2302 Bioquímica
2415 Biología Molecular
Palabras Clave
Salsola soda
Cytotoxicity
Citotoxicidad
ISSN
2072-6651
Revisión por pares
SI
Patrocinador
University of Campania ‘Luigi Vanvitelli"- (project ARS01_01166)
Junta de Castilla y León, Consejería de Educación - (Grant VA033G19)
Junta de Castilla y León, Consejería de Educación - (Grant VA033G19)
Version del Editor
Propietario de los Derechos
© 2022 The Authors
Idioma
eng
Tipo de versión
info:eu-repo/semantics/publishedVersion
Derechos
openAccess
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