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Título
FGF2/EGF contributes to brain neuroepithelial precursor proliferation and neurogenesis in rat embryos: the involvement of embryonic cerebrospinal fluid
Autor
Año del Documento
2019
Editorial
Wiley
Descripción
Producción Científica
Documento Fuente
Developmental Dynamics, 2020, vol. 249, n. 1, p. 141-153
Zusammenfassung
Background: At the earliest stages of brain development, the neuroepithelium
works as an interdependent functional entity together with cerebrospinal fluid,
which plays a key regulatory role in neuroepithelial cell survival, replication
and neurogenesis; however, the underlying mechanism remains unknown in
mammals.
Results: We show the presence of fibroblast growth factor 2 (FGF2) and epidermal
growth factor (EGF), in 13.5-day rat embryo cerebrospinal fluid (eCSF).
Immunohistochemical detection of FGF2 expression localized this factor inside
neuroepithelial precursors close to the neuroepithelial-CSF interface,
suggesting that FGF2 from eCSF could originate in the neuroepithelium by
apical secretion. The colocalization of FGFR1 and FGF2 in some neuroepithelial
cells close to the ventricular surface suggests they are target cells
for eCSF FGF2. Brain neuroepithelium EGF expression was negative. By using
a neuroepithelial organotypic culture, we demonstrate that FGF2 and EGF
from eCSF plays a specific role in triggering the self-renewal and are involved
in neurogenetic induction of mesencephalic neuroepithelial precursor cells
during rat development.
Conclusions: We propose eCSF as an intercommunication medium for neuroepithelial
precursor behavior control during early rat brain development, and
the neuroepithelial regulation of FGF2 and EGF presence in eCSF, as a regulative
mechanism controlling precursor proliferation and neurogenesis.
Materias (normalizadas)
Neurología del desarrollo
Palabras Clave
EGF
FGF2
Precursores neuronales
Mitogénesis
Neurogénesis
ISSN
1058-8388
Revisión por pares
SI
DOI
Patrocinador
Junta de Castilla y León, Consejería de educación, Grant/Award Number: GR195; Ministerio de Educación y Ciencia, Grant/ Award Number: BFU207/6516
Version del Editor
Idioma
spa
Tipo de versión
info:eu-repo/semantics/publishedVersion
Derechos
openAccess
Aparece en las colecciones
Dateien zu dieser Ressource
Tamaño:
2.212Mb
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Adobe PDF
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