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dc.contributor.authorCitores González, Lucía 
dc.contributor.authorRagucci, Sara
dc.contributor.authorFerreras Rodríguez, José Miguel 
dc.contributor.authorDi Maro, Antimo
dc.contributor.authorIglesias Álvarez, María del Rosario 
dc.date.accessioned2024-01-31T19:56:21Z
dc.date.available2024-01-31T19:56:21Z
dc.date.issued2019
dc.identifier.citationACS Chemical Biology, May 2019, vol. 14 (6), p. 1319-1327es
dc.identifier.issn1554-8929es
dc.identifier.urihttps://uvadoc.uva.es/handle/10324/65470
dc.descriptionProducción Científicaes
dc.description.abstractRibotoxins make up a group of extracellular rRNA endoribonucleases produced by ascomycetes that display cytotoxicity toward animal cells, having been proposed as insecticidal agents. Recently, the ribotoxin Ageritin has been isolated from the basidiomycetes Agrocybe aegerita (poplar mushroom), suggesting that ribotoxins are widely distributed among fungi. To gain insights into the protective properties of Ageritin against pathogens and its putative biotechnological applications, we have tested several biological activities of Ageritin, comparing them with those of the well-known ribotoxin α-sarcin, and we found that Ageritin displayed, in addition to the already reported activities, (i) antibacterial activity against Micrococcus lysodeikticus, (ii) activity against the tobacco mosaic virus RNA, (iii) endonuclease activity against a supercoiled plasmid, (iv) nuclease activity against genomic DNA, (v) cytotoxicity to COLO 320, HeLa, and Raji cells by promoting apoptosis, and (vi) antifungal activity against the green mold Penicillium digitatum. Therefore, Ageritin and α-sarcin can induce resistance not only to insects but also to viruses, bacteria, and fungi. The multiple biological activities of Ageritin could be exploited to improve resistance to different pathogens by engineering transgenic plants. Furthermore, the induction of cell death by different mechanisms turns these ribotoxins into useful tools for cancer therapy.es
dc.format.mimetypeapplication/pdfes
dc.language.isoenges
dc.publisherAmerican Chemical Societyes
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subject.classificationribotoxines
dc.subject.classificationgreen mouldes
dc.subject.classificationantifungal agentes
dc.subject.classificationribosome inactivationes
dc.subject.classificationapoptosises
dc.subject.classificationRibosome-inactivating proteines
dc.subject.classificationCyclocybe aegeritaes
dc.subject.classificationageritines
dc.titleAgeritin, a Ribotoxin from Poplar Mushroom (Agrocybe aegerita) with Defensive and Antiproliferative Activitieses
dc.typeinfo:eu-repo/semantics/articlees
dc.rights.holderCopyright © 2019 American Chemical Societyes
dc.identifier.doi10.1021/acschembio.9b00291es
dc.relation.publisherversionhttps://pubs.acs.org/doi/10.1021/acschembio.9b00291es
dc.identifier.publicationfirstpage1319es
dc.identifier.publicationissue6es
dc.identifier.publicationlastpage1327es
dc.identifier.publicationtitleACS Chemical Biologyes
dc.identifier.publicationvolume14es
dc.peerreviewedSIes
dc.description.projectConsejería de Sanidad de la Junta de Castilla y León Regional Grant BIO/VA39/14es
dc.description.project“Progetti per la ricerca oncologica della Regione Campania”, named Grant I-Curees
dc.identifier.essn1554-8937es
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.type.hasVersioninfo:eu-repo/semantics/acceptedVersiones
dc.subject.unesco2302 Bioquímicaes
dc.subject.unesco2302.27 Proteínases
dc.subject.unesco2302.21 Biología Moleculares
dc.subject.unesco3108.05 Hongoses
dc.subject.unesco3214 Toxicologíaes


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