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dc.contributor.authorVega-Agapito, Victoria
dc.contributor.authorAlmeida, Angeles
dc.contributor.authorHatzoglou, Maria
dc.contributor.authorBolaños, Juan P
dc.date.accessioned2024-02-02T23:14:37Z
dc.date.available2024-02-02T23:14:37Z
dc.date.issued2002-08-16
dc.identifier.citationThe Journal of Biolgical Chemistry, agosto 16, 277, 33, 29753-29759es
dc.identifier.urihttps://uvadoc.uva.es/handle/10324/65608
dc.descriptionProducción Científicaes
dc.description.abstractWe have reported previously that peroxynitrite stimulates L-arginine release from astrocytes, but the mechanism responsible for such an effect remains elusive. To explore this issue, we studied the regulation of L-[3 H]arginine transport by either exogenous or endogenous peroxynitrite in glial cells. A 2-fold peroxynitritemediated stimulation of L-arginine release in C6 cells was found to be Na -independent, was prevented by 5 mM L-arginine and, although only in the presence of Na , was blocked by 5 mM L-alanine or L-leucine. Peroxynitrite-mediated stimulation of L-arginine uptake was trans-stimulated by 10 mM L-arginine and was inhibited in a dose-dependent fashion (ki of 40 M) by the system y inhibitor N-ethylmaleimide in C6 cells. Endogenous production of peroxynitrite in lipopolysaccharidetreated astrocytes triggered an increased L-arginine transport activity without affecting Cat1 L-arginine transporter mRNA levels. However, Western blot analyses of peroxynitrite-treated astrocytes and C6 glial cells revealed a 3-nitrotyrosinated anti-Cat1-immunopositive band, strongly suggesting peroxynitrite-mediated Cat1 nitration. Furthermore, peroxynitrite stimulation of Larginine release was abolished in fibroblast cells homozygous for a targeted inactivation of the Cat1 gene. Finally, peroxynitrite-triggered L-arginine released from astrocytes was efficiently taken up by neurons in an insert-based co-culture system. These results strongly suggest that peroxynitrite-mediated activation of the Cat1 transporter in glial cells may serve as a mechanism focused to replenish L-arginine in the neighboring neurons.es
dc.format.mimetypeapplication/pdfes
dc.language.isoenges
dc.publishery The American Society for Biochemistry and Molecular Biology, Inces
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.titlePeroxynitrite Stimulates L-Arginine Transport System y+ in Glial Cells A POTENTIAL MECHANISM FOR REPLENISHING NEURONAL L-ARGININEes
dc.typeinfo:eu-repo/semantics/articlees
dc.identifier.doihttps://doi.org/10.1074/jbc.M203728200es
dc.relation.publisherversionhttps://www.jbc.org/action/showPdf?pii=S0021-9258%2818%2975638-Xes
dc.identifier.publicationfirstpage29753es
dc.identifier.publicationissue33es
dc.identifier.publicationlastpage29759es
dc.identifier.publicationvolume277es
dc.peerreviewedSIes
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersiones


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