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    • Dpto. Bioquímica y Biología Molecular y Fisiología
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    Por favor, use este identificador para citar o enlazar este ítem:https://uvadoc.uva.es/handle/10324/65829

    Título
    Transcriptional Basis of Ca2+ Remodeling Reversal Induced by Polyamine Synthesis Inhibition in Colorectal Cancer Cells
    Autor
    Pérez-Riesgo, Enrique
    Hernando Pérez, María ElenaAutoridad UVA Orcid
    Feijóo, Verónica
    Tajada Esteban, SendoaAutoridad UVA
    Núñez Llorente, LucíaAutoridad UVA
    Villalobos, Carlos
    Año del Documento
    2023
    Documento Fuente
    Cancers (Basel). 2023 Mar 4;15(5):1600.
    Resumen
    Colorectal cancer (CRC) is associated with mutations in APC/Wnt leading to c-myc activation and the overexpression of ODC1, the limiting step in polyamine synthesis. CRC cells also display a remodeling of intracellular Ca2+ homeostasis that contributes to cancer hallmarks. As polyamines may modulate Ca2+ homeostasis during epithelial tissue repair, we investigated whether polyamine synthesis inhibition may reverse Ca2+ remodeling in CRC cells and, if so, the molecular basis for this reversal. To this end, we used calcium imaging and transcriptomic analysis in normal and CRC cells treated with DFMO, an ODC1 suicide inhibitor. We found that polyamine synthesis inhibition partially reversed changes in Ca2+ homeostasis associated with CRC, including a decrease in resting Ca2+ and SOCE along with an increased Ca2+ store content. We also found that polyamine synthesis inhibition reversed transcriptomic changes in CRC cells without affecting normal cells. Specifically, DFMO treatment enhanced the transcription of SOCE modulators CRACR2A; ORMDL3; and SEPTINS 6, 7, 8, 9, and 11, whereas it decreased SPCA2, involved in store-independent Orai1 activation. Therefore, DFMO treatment probably decreased store-independent Ca2+ entry and enhanced SOCE control. Conversely, DFMO treatment decreased the transcription of the TRP channels TRPC1 and 5, TRPV6, and TRPP1 while increasing TRPP2, thus probably decreasing Ca2+ entry through TRP channels. Finally, DFMO treatment enhanced the transcription of the PMCA4 Ca2+ pump and mitochondrial channels MCU and VDAC3 for enhanced Ca2+ extrusion through the plasma membrane and mitochondria. Collectively, these findings suggested the critical role of polyamines in Ca2+ remodeling in colorectal cancer.
    Revisión por pares
    SI
    DOI
    10.3390/cancers15051600
    Idioma
    spa
    URI
    https://uvadoc.uva.es/handle/10324/65829
    Tipo de versión
    info:eu-repo/semantics/draft
    Derechos
    openAccess
    Aparece en las colecciones
    • DEP06 - Artículos de revista [352]
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    Nombre:
    Pérez-Riesgo et al. - 2023 - Transcriptional Basis of Ca2 Remodeling Reversal Induced by Polyamine Synthesis Inhibition in Colorectal Ca.pdf
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