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dc.contributor.authorDinger, Bruce
dc.contributor.authorGonzález, Constancio
dc.contributor.authorYoshizaki, Katsuaki
dc.contributor.authorFidone, Salvatore
dc.date.accessioned2014-10-30T13:15:55Z
dc.date.available2014-10-30T13:15:55Z
dc.date.issued1985
dc.identifier.citationBrain Research, 1985, vol. 339, p. 295-304es
dc.identifier.issn0006-8993es
dc.identifier.urihttp://uvadoc.uva.es/handle/10324/6845
dc.descriptionProducción Científicaes
dc.description.abstractAcetylcholine and nicotinic agents excite cat carotid body chemoreceptors and modify their response to natural stimuli. The present experiments utilized [125I]a-bungarotoxin ([125I]a-BGT) to localize within the chemosensory tissue the possible sites of action of exogenous and endogenous nicotinic cholinergic substances. In vitro equilibrium binding studies of intact carotid bodies determined a K d of 5.57 nM and a Bma x of 9.21 pmol/g of tissue. Chronic section (12-15 days) of the carotid sinus nerve (CSN) did not change the amount of displaceable toxin binding. In contrast, the specific binding was reduced by 46% following removal of the superior cervical ganglion. Light microscope autoradiography of normal, CSN-denervated and sympathectomized carotid bodies revealed displaceable binding sites concentrated in lobules of type I and type II cells. Treatment of carotid bodies with 50 nM a-BGT in vitro reduced by 50% the release of [3H]dopamine (synthesized from [3H]tyrosine) caused by hypoxia or nicotine, and also significantly reduced the stimulus-. evoked discharges recorded from the CSN. The data suggest (1) an absence of ct-BGT binding sites on the afferent terminals of the CSN and (2) that nicotinic receptors located within parenchymal cell lobules may modulate the release of catecholamines from these cells.es
dc.format.mimetypeapplication/pdfes
dc.language.isoenges
dc.publisherElsevieres
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectNeurofisiologíaes
dc.titleLocalization and Function of Cat Carotid Body Nicotinic Receptorses
dc.typeinfo:eu-repo/semantics/articlees
dc.identifier.publicationfirstpage295es
dc.identifier.publicationlastpage304es
dc.identifier.publicationtitleBrain Researches
dc.identifier.publicationvolume339es
dc.peerreviewedSIes
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International


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