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dc.contributor.authorSarisoy, Alp
dc.contributor.authorAcosta Rodríguez, Sergio 
dc.contributor.authorRodríguez Cabello, José Carlos 
dc.contributor.authorCzichowski, Phillip
dc.contributor.authorKopp, Alexander
dc.contributor.authorJockenhoevel, Stefan
dc.contributor.authorFernández Colino, Alicia
dc.date.accessioned2024-07-26T08:43:35Z
dc.date.available2024-07-26T08:43:35Z
dc.date.issued2023
dc.identifier.citationInternational Journal of Molecular Sciences, 2023, Vol. 24, Nº. 7, 6776es
dc.identifier.issn1422-0067es
dc.identifier.urihttps://uvadoc.uva.es/handle/10324/69182
dc.descriptionProducción Científicaes
dc.description.abstractMore than 260 million surgical procedures are performed worldwide each year. Although sutures and staples are widely used to reconnect tissues, they can cause further damage and increase the risk of infection. Bioadhesives have been proposed as an alternative to reconnect tissues. However, clinical adhesives that combine strong adhesion with cytocompatibility have yet to be developed. In this study, we explored the production of adhesives based on protein-engineered polymers bioinspired by the sequence of elastin (i.e., elastin-like recombinamers, ELRs). We hypothesized that the combination of polyphenols (i.e., tannic acid, TA) and ELRs would produce an adhesive coacervate (ELR+TA), as reported for other protein polymers such as silk fibroin (SF). Notably, the adhesion of ELR alone surpassed that of ELR+TA. Indeed, ELR alone achieved adhesive strengths of 88.8 ± 33.2 kPa and 17.0 ± 2.0 kPa on porcine bone and skin tissues, respectively. This surprising result led us to explore a multicomponent bioadhesive to encompass the complementary roles of elastin (mimicked here by ELR) and silk fibroin (SF), and subsequently mirror more closely the multicomponent nature of the extracellular matrix. Tensile testing showed that ELR+SF achieved an adhesive strength of 123.3 ± 60.2 kPa on porcine bone and excellent cytocompatibility. To express this in a more visual and intuitive way, a small surface of only 2.5 cm2 was able to lift at least 2 kg of weight. This opens the door for further studies focusing on the ability of protein-engineered polymers to adhere to biological tissues without further chemical modification for applications in tissue engineering.es
dc.format.mimetypeapplication/pdfes
dc.language.isoenges
dc.publisherMDPIes
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectAdhesiveses
dc.subjectAdhesivoses
dc.subjectBiomaterialses
dc.subjectBiomaterialeses
dc.subjectBiomedical materialses
dc.subjectPolymerses
dc.subjectProteinses
dc.subjectMedicinees
dc.subjectPolímeros recombinanteses
dc.subjectTejidos (Histología)es
dc.subjectBoneses
dc.subjectHuesoses
dc.subjectSkines
dc.subjectPieles
dc.subjectOrganic chemistryes
dc.subjectInorganic chemistryes
dc.subjectMolecular biologyes
dc.subjectMedical technologyes
dc.titleBioglues based on an elastin-like recombinamer: effect of tannic acid as an additive on tissue adhesion and cytocompatibilityes
dc.typeinfo:eu-repo/semantics/articlees
dc.rights.holder© 2023 The authorses
dc.identifier.doi10.3390/ijms24076776es
dc.relation.publisherversionhttps://www.mdpi.com/1422-0067/24/7/6776es
dc.identifier.publicationfirstpage6776es
dc.identifier.publicationissue7es
dc.identifier.publicationtitleInternational Journal of Molecular Scienceses
dc.identifier.publicationvolume24es
dc.peerreviewedSIes
dc.description.projectGobiernos federal y estatal de Alemania - (StUpPD370-20)es
dc.description.projectMinisterio Alemán de Educación e Investigación (BMBF) - (grant 13XP5136)es
dc.description.projectGobierno de España - (grant PID2019- 110709RB-100, RED2018-102417-T)es
dc.description.projectJunta de Castilla y León - (grant VA317P18, Infrared2018-UVA06)es
dc.description.projectPrograma de Cooperación Interreg España-Portugal POCTEP - (grant 0624_2IQBIONEURO_6_E)es
dc.identifier.essn1422-0067es
dc.rightsAtribución 4.0 Internacional*
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersiones
dc.subject.unesco2206.10 Polímeroses
dc.subject.unesco2302.27 Proteínases
dc.subject.unesco2306 Química Orgánicaes
dc.subject.unesco2303 Química Inorgánicaes
dc.subject.unesco32 Ciencias Médicases
dc.subject.unesco2415 Biología Moleculares
dc.subject.unesco3314 Tecnología Médicaes


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