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Título
Protective actions of α-tocopherol on cell membrane lipids of paraquat-stressed human astrocytes using microarray technology, MALDI-MS and lipidomic analysis
Autor
Año del Documento
2022
Editorial
MDPI
Descripción
Producción Científica
Documento Fuente
Antioxidants, 2022, Vol. 11, Nº. 12, 2440
Abstract
Cellular senescence is one of the main contributors to some neurodegenerative disorders. The early detection of senescent cells or their related effects is a key aspect in treating disease progression. In this functional deterioration, oxidative stress and lipid peroxidation play an important role. Endogenous antioxidant compounds, such as α-tocopherol (vitamin E), can mitigate these undesirable effects, particularly lipid peroxidation, by blocking the reaction between free radicals and unsaturated fatty acid. While the antioxidant actions of α-tocopherol have been studied in various systems, monitoring the specific effects on cell membrane lipids at scales compatible with large screenings has not yet been accomplished. Understanding the changes responsible for this protection against one of the consequences of senescence is therefore necessary. Thus, the goal of this study was to determinate the changes in the lipid environment of a Paraquat-treated human astrocytic cell line, as a cellular oxidative stress model, and the specific actions of the antioxidant, α-tocopherol, using cell membrane microarray technology, MALDI-MS and lipidomic analysis. The stress induced by Paraquat exposure significantly decreased cell viability and triggered membrane lipid changes, such as an increase in certain species of ceramides that are lipid mediators of apoptotic pathways. The pre-treatment of cells with α-tocopherol mitigated these effects, enhancing cell viability and modulating the lipid profile in Paraquat-treated astrocytes. These results demonstrate the lipid modulation effects of α-tocopherol against Paraquat-promoted oxidative stress and validate a novel analytical high-throughput method combining cell cultures, microarray technology, MALDI-MS and multivariate analysis to study antioxidant compounds against cellular senescence.
Materias (normalizadas)
Oxidative stress
Estrés oxidativo
Lipids
Lípidos
Lipids - Peroxidation
Microarrays
DNA microarrays
ADN
Cells - Aging
Aging
Envejecimiento
Astrocytes
Astrocitos
Genetics
Genética
Biochemistry
Clinical biochemistry
Bioquímica clínica
Cell biology
Materias Unesco
2302 Bioquímica
2407 Biología Celular
ISSN
2076-3921
Revisión por pares
SI
Patrocinador
Ministerio de Economía y Competitividad - (grants DIN2019-010902 y PTQ 17-09409)
Ministerio de Ciencia, Innovación y Universidades/Agencia Estatal de Investigación (AEI)/10.13039/501100011033 - (grant PID2019-110911RB-I00)
Ministerio de Ciencia, Innovación y Universidades/Agencia Estatal de Investigación (AEI)/10.13039/501100011033 - (grant PID2019-110911RB-I00)
Version del Editor
Propietario de los Derechos
© 2022 The authors
Idioma
eng
Tipo de versión
info:eu-repo/semantics/publishedVersion
Derechos
openAccess
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