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    Por favor, use este identificador para citar o enlazar este ítem:https://uvadoc.uva.es/handle/10324/74260

    Título
    Requirement of JNK-mediated phosphorylation for translocation of group IVA phospholipase A2 to phagosomes in human macrophages
    Autor
    Casas Requena, JavierAutoridad UVA Orcid
    Meana González, ClaraAutoridad UVA Orcid
    Esquinas, Esperanza
    Valdearcos, Martín
    Pindado, José
    Balsinde Rodríguez, Jesús
    Balboa García, María Ángeles
    Año del Documento
    2009
    Documento Fuente
    Journal of Immunology, Agosto 2009, vol. 183, n. 4, p. 2767-2774
    Resumo
    Eicosanoids are a broad family of lipids that play a critical role in host defense against bacterial and fungal infections. The first enzyme in the metabolic pathway for the generation of eicosanoids is group IVA phospholipase A2, also known as cytosolic phospholipase A2 (cPLA2 ). During phagocytosis, cPLA2 has been found to translocate to the phagosome, although the molecular mechanism involved in such a translocation has not been elucidated. By using enhanced GFP-tagged proteins we show in this work that a nonphosphorylatable cPLA2 mutant (S505A) does not translocate to the phagosomes, but a mutant that mimics phosphorylation on Ser505 (S505E) does it so readily. During phagocytosis, endogenous cPLA2 is phosphorylated at Ser505, and inhibitors of JNK, but not of other related kinases such as p38 or the extracellular-regulated kinases 1 and 2, completely block such a phosphorylation. Inhibition of JNK activity also inhibits the translocation of cPLA2 to phagosomal membranes, as well as arachidonic acid release to the extracellular medium. Moreover, the S505E mutant makes the enzyme refractory to JNK inhibition, translocating normally to phagosomal membranes. Collectively, these data support a key role for JNK-mediated cPLA2 phosphorylation at Ser505 in the sequence of events leading to translocation and activation of the enzyme to phagosomal membranes in human macrophages.
    ISSN
    0022-1767
    Revisión por pares
    SI
    DOI
    10.4049/jimmunol.0901530
    Version del Editor
    https://journals.aai.org/jimmunol/article/183/4/2767/83781/Requirement-of-JNK-Mediated-Phosphorylation-for
    Idioma
    eng
    URI
    https://uvadoc.uva.es/handle/10324/74260
    Tipo de versión
    info:eu-repo/semantics/publishedVersion
    Derechos
    openAccess
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    • DEP06 - Artículos de revista [353]
    • DEP55 - Artículos de revista [209]
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    07. 2009, JI. Requirement of JNK-mediated phosphorylation for translocation of gIVA PLA2 to phagosomes in human MQ.pdf
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    Universidad de Valladolid

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