• español
  • English
  • français
  • Deutsch
  • português (Brasil)
  • italiano
    • español
    • English
    • français
    • Deutsch
    • português (Brasil)
    • italiano
    • español
    • English
    • français
    • Deutsch
    • português (Brasil)
    • italiano
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Parcourir

    Tout UVaDOCCommunautésPar date de publicationAuteursSujetsTitres

    Mon compte

    Ouvrir une session

    Statistiques

    Statistiques d'usage de visualisation

    Compartir

    Voir le document 
    •   Accueil de UVaDOC
    • PUBLICATIONS SCIENTIFIQUES
    • Research Data
    • Datasets
    • Voir le document
    •   Accueil de UVaDOC
    • PUBLICATIONS SCIENTIFIQUES
    • Research Data
    • Datasets
    • Voir le document
    • español
    • English
    • français
    • Deutsch
    • português (Brasil)
    • italiano

    Exportar

    RISMendeleyRefworksZotero
    • edm
    • marc
    • xoai
    • qdc
    • ore
    • ese
    • dim
    • uketd_dc
    • oai_dc
    • etdms
    • rdf
    • mods
    • mets
    • didl
    • premis

    Citas

    Por favor, use este identificador para citar o enlazar este ítem:https://uvadoc.uva.es/handle/10324/78598

    Título
    Aqueous two-phase systems based on natural deep eutectic solvents for protein extraction from microalgal biomass grown in wastewater
    Autor
    Moldes Plaza, DavidAutoridad UVA Orcid
    Vega Alegre, María del SolAutoridad UVA Orcid
    Bolado Rodríguez, SilviaAutoridad UVA Orcid
    Fernández Requejo, PatriciaAutoridad UVA Orcid
    Editor
    Universidad de Valladolid. Instituto de Procesos Sostenibles
    Año del Documento
    2025
    Résumé
    The cultivation of microalgae in photobioreactors offers a sustainable approach for efficient treatment of nutrient-rich wastewater while producing biomass rich in valuable biocompounds that can be recovered. Aqueous two-phase systems (ATPSs) composed of the combination of two natural deep eutectic solvents (NADESs), betaine-levulinic acid (Bet:2LA) and choline chloride-urea (ChCl:2Urea), with di- and tripotassium phosphate salts, were evaluated as green extraction technologies for protein recovery from microalgal biomass cultivated in swine wastewater. The extraction capacity was compared with that of ATPSs formed by natural metabolites betaine or choline chloride and the phosphate salts. Binodal curves and tie-lines were experimentally determined for each system at 25 ºC to characterize the ATPS studied. Protein recovery yield (PRY) from the biomass was analyzed for the six ATPSs at three different extraction times at 25 ºC. The best compromise between PRY, selectivity relative to carbohydrate coextraction, and extraction time was achieved using the Bet:2LA NADES paired with K3PO4 after 30 min (PRY 16.4 %). This ATPS contains 50 % water, underscoring its environmentally friendly profile. These findings suggest the feasibility of NADES-based ATPS for efficient and fast protein extraction from wastewater-derived microalgal biomass in mild conditions.  
    Palabras Clave
    ATPs
    designer solvents
    green extraction
    protein-carbohydrate separation
    residues valorization
    Scenedesmus Almeriensis
    swine wastewater
    Departamento
    Instituto de Procesos Sostenibles
    Departamento de Química Analítica
    Departamento de Ingeniería Química y Tecnología del Medio Ambiente
    Patrocinador
    Ministerio de Ciencia, Innovación y Universidades
    Consejeria de Educacion Junta de Castilla y Leon
    EU Feder Programme
    MICIU/AEI/ 10.13039/501100011033
    Patrocinador
    PID2023-153356OB-I00
    FPU20/02086
    UIC 338
    CLU-2025-2-06
    Idioma
    eng
    URI
    https://uvadoc.uva.es/handle/10324/78598
    Tipo de versión
    info:eu-repo/semantics/draft
    Derechos
    openAccess
    Aparece en las colecciones
    • Datasets [90]
    Afficher la notice complète
    Fichier(s) constituant ce document
    Nombre:
    Dataset paper Scenedesmus ATPS_MJ .xlsx
    Tamaño:
    159.2Ko
    Formato:
    Hoja Excel
    Voir/Ouvrir
    CC0 1.0 UniversalExcepté là où spécifié autrement, la license de ce document est décrite en tant que CC0 1.0 Universal

    Universidad de Valladolid

    Powered by MIT's. DSpace software, Version 5.10