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Por favor, use este identificador para citar o enlazar este ítem: http://uvadoc.uva.es/handle/10324/21809
Título: In vitro aging promotes endoplasmic reticulum (ER)-mitochondria Ca2+ cross talk and loss of store-operated Ca2+ entry (SOCE) in rat hippocampal neurons
Autor: Calvo Rodríguez, María
García Durillo, Mónica
Villalobos, Carlos
Nuñez Llorente, Lucía
Año del Documento: 2016
Editorial: Elsevier
Descripción: Producción Científica
Documento Fuente: Biochim Biophys Acta Mol Cel Res 1863(11): 2637-2649
Resumen: Aging is associated to cognitive decline and susceptibility to neuron death, two processes related recently to subcellular Ca2+ homeostasis. Memory storage relies on mushroom spines stability that depends on store-operated Ca2+ entry (SOCE). In addition, Ca2+ transfer from endoplasmic reticulum(ER) to mitochondria sustains energy production but mitochondrial Ca2+ overload promotes apoptosis. We have addressed whether SOCE and ERmitochondria Ca2+ transfer are influenced by culture time in long-term cultures of rat hippocampal neurons, a model of neuronal aging.We found that short-term cultured neurons show large SOCE, low Ca2+ store content and no functional coupling between ER and mitochondria. In contrast, in long-term cultures reflecting aging neurons, SOCE is essentially lost, Stim1 and Orai1 are downregulated, Ca2+ stores becomeoverloaded, Ca2+ release is enhanced, expression of the mitochondrial Ca2+ uniporter (MCU) increases and most Ca2+ released from the ER is transferred to mitochondria. These results suggest that neuronal aging is associated to increased ERmitochondrial cross talking and loss of SOCE. This subcellular Ca2+ remodeling might contribute to cognitive decline and susceptibility to neuron cell death in the elderly.
Materias (normalizadas): Hippocampal neurons
ISSN: 1388-1981
Revisión por Pares: SI
DOI: 10.1016/j.bbamcr.2016.08.001
Patrocinador: Ministerio de Economía y Competitividad (BFU2012-37146 and BFU2015- 70131R)
Junta de Castilla y León (VA145U13 and BIO/VA33/13)
Version del Editor: http://www.journals.elsevier.com/bba-molecular-and-cell-biology-of-lipids/
Idioma: eng
URI: http://uvadoc.uva.es/handle/10324/21809
Derechos: info:eu-repo/semantics/openAccess
Aparece en las colecciones:DEP06 - Artículos de revista

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