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Title: In vitro aging promotes endoplasmic reticulum (ER)-mitochondria Ca2+ cross talk and loss of store-operated Ca2+ entry (SOCE) in rat hippocampal neurons
Authors: Calvo Rodríguez, María
García Durillo, Mónica
Villalobos, Carlos
Nuñez Llorente, Lucía
Issue Date: 2016
Publisher: Elsevier
Description: Producción Científica
Citation: Biochim Biophys Acta Mol Cel Res 1863(11): 2637-2649
Abstract: Aging is associated to cognitive decline and susceptibility to neuron death, two processes related recently to subcellular Ca2+ homeostasis. Memory storage relies on mushroom spines stability that depends on store-operated Ca2+ entry (SOCE). In addition, Ca2+ transfer from endoplasmic reticulum(ER) to mitochondria sustains energy production but mitochondrial Ca2+ overload promotes apoptosis. We have addressed whether SOCE and ERmitochondria Ca2+ transfer are influenced by culture time in long-term cultures of rat hippocampal neurons, a model of neuronal aging.We found that short-term cultured neurons show large SOCE, low Ca2+ store content and no functional coupling between ER and mitochondria. In contrast, in long-term cultures reflecting aging neurons, SOCE is essentially lost, Stim1 and Orai1 are downregulated, Ca2+ stores becomeoverloaded, Ca2+ release is enhanced, expression of the mitochondrial Ca2+ uniporter (MCU) increases and most Ca2+ released from the ER is transferred to mitochondria. These results suggest that neuronal aging is associated to increased ERmitochondrial cross talking and loss of SOCE. This subcellular Ca2+ remodeling might contribute to cognitive decline and susceptibility to neuron cell death in the elderly.
Keywords: Hippocampal neurons
ISSN: 1388-1981
Peer Review: SI
DOI: 10.1016/j.bbamcr.2016.08.001
Sponsor: Ministerio de Economía y Competitividad (BFU2012-37146 and BFU2015- 70131R)
Junta de Castilla y León (VA145U13 and BIO/VA33/13)
Publisher Version:
Language: eng
Rights: info:eu-repo/semantics/openAccess
Appears in Collections:DEP06 - Artículos de revista

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