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Título: A new procedure for amyloid oligomers preparation enables the unambiguous testing of their effects on cytosolic and mitochondrial Ca2+ entry and cell death in primary neurons
Autor: Caballero, Erica
Calvo Rodríguez, María
Gonzalo Ruiz, Alicia
Villalobos, Carlos
Nuñez Llorente, Lucía
Año del Documento: 2016
Editorial: Elsevier
Descripción: Producción Científica
Documento Fuente: Neuroscience Letters Volume 612, 26 January 2016, Pages 66–73
Resumen: Oligomers of the amyloid peptide (A o) are becoming the most likely neurotoxin in Alzheimer’s disease. Controversy remains on the mechanisms involved in neurotoxicity induced by A o and the targets involved. We have reported that A o promote Ca2+ entry, mitochondrial Ca2+ overload and apoptosis in cultured cerebellar neurons. However, recent evidence suggests that some of these effects could be induced by glutamate receptor agonists solved in F12, the media in which A o are prepared. Here we have tested the effects of different media on A o formation and on cytosolic Ca2+ concentration ([Ca2+]cyt) in rat cerebellar and hippocampal cell cultures. We found that A o prepared according to previous protocols but solved in alternative media including saline, MEM and DMEM do not allow oligomer formation and fail to increase [Ca2+]cyt. Changes in the oligomerization protocol and supplementation of media with selected salts reported to favor oligomer formation enable A o formation. A o prepared by the new procedure and containing small molecular weight oligomers increased [Ca2+]cyt, promoted mitochondrial Ca2+ overload and cell death in cerebellar granule cells and hippocampal neurons. These results foster a role for Ca2+ entry in neurotoxicity induced by A o and provide a reliable procedure for investigating the Ca2+ entry pathway promoted by A o.
Materias (normalizadas): Alzheimer
ISSN: 0304-3940
Revisión por Pares: SI
DOI: 10.1016/j.neulet.2015.11.041
Patrocinador: Ministerio de Economía y Competitividad (BFU2012-37146)
Junta de Castilla y León (VA145U13, BIO/VA33/13 and BIO103/VA45/11)
Version del Editor:
Idioma: eng
Derechos: info:eu-repo/semantics/openAccess
Aparece en las colecciones:DEP06 - Artículos de revista

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