|Título: ||Elastin-like recombinamers with acquired functionalities for gene-delivery applications|
|Autor: ||Piña, M. J.|
Susan, M. Alex
Arias Vallejo, F. J.
Santos García, M.
Rodríguez Cabello, José Carlos
Mannemcherril, R. R.
Sharma, Chandra P.
|Año del Documento: ||2015|
|Editorial: ||Society for Biomaterials|
|Descripción: ||Producción Científica|
|Documento Fuente: ||J Biomed Mater Res A. 2015 Oct;103(10):3166-78|
|Resumen: ||In this work, well-defined elastin-like recombinamers (ELRs) were studied as a choice to the existing nonviral vectors due to their biocompatibility and ease of scale-up. Functional motifs, namely penetratin and LAEL fusogenic peptides were incorporated into a basic ELR sequence, and imidazole groups were subsequently covalently bound obtaining ELRs with new functionalities. Stable polyplexes composed of plasmid DNA and ELRs were formed. A particle size around 200 nm and a zeta potential up to nearly +24 mV made them suitable for gene delivery purposes. Additionally, viability and transfection assays with C6 rat glioma cell line showed an increase in the cellular uptake and transfection levels for the construction containing the LAEL motif. This study highlights the importance of controlling the polymer functionality using recombinant techniques and establishes the utility of ELRs as biocompatible nonviral systems for gene-therapy applications.|
|Palabras Clave: ||ELR|
|Revisión por Pares: ||NO|
|DOI: ||DOI: 10.1002/jbm.a.35455|
|Patrocinador: ||ERDF Funding from the EU and MINECO; contract grant number: MAT2010-15982|
FCCI Subprogram: Modality ACI-COLABORA Spain-India 2010-2012; contract grant numbers: MAT2010-15310, PRIPIBAR- 2011-1403, MAT2012-38043, and MAT2013-41723-R
JCyL; contract grant numbers: VA049A11, VA152A12, and VA155A12
CIBER-BBN, JCyL, and the Instituto de Salud Carlos III under the “Network Center of Regenerative Medicine and Cellular Therapy of Castilla and Leon” and DST (Indo-Spanish), (New Delhi)
|Aparece en las colecciones:||BIOFORGE - Artículos de revista|