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Por favor, use este identificador para citar o enlazar este ítem: http://uvadoc.uva.es/handle/10324/22962
Título: Elastin-like recombinamers with acquired functionalities for gene-delivery applications
Autor: Piña, M. J.
Susan, M. Alex
Arias Vallejo, F. J.
Santos García, M.
Rodríguez Cabello, José Carlos
Mannemcherril, R. R.
Sharma, Chandra P.
Año del Documento: 2015
Editorial: Society for Biomaterials
Descripción: Producción Científica
Documento Fuente: J Biomed Mater Res A. 2015 Oct;103(10):3166-78
Resumen: In this work, well-defined elastin-like recombinamers (ELRs) were studied as a choice to the existing nonviral vectors due to their biocompatibility and ease of scale-up. Functional motifs, namely penetratin and LAEL fusogenic peptides were incorporated into a basic ELR sequence, and imidazole groups were subsequently covalently bound obtaining ELRs with new functionalities. Stable polyplexes composed of plasmid DNA and ELRs were formed. A particle size around 200 nm and a zeta potential up to nearly +24 mV made them suitable for gene delivery purposes. Additionally, viability and transfection assays with C6 rat glioma cell line showed an increase in the cellular uptake and transfection levels for the construction containing the LAEL motif. This study highlights the importance of controlling the polymer functionality using recombinant techniques and establishes the utility of ELRs as biocompatible nonviral systems for gene-therapy applications.
Palabras Clave: ELR
Elastin-like Recombinamers
Gene therapy
Cationic Polymers
Polyplex
Revisión por Pares: NO
DOI: DOI: 10.1002/jbm.a.35455
Patrocinador: ERDF Funding from the EU and MINECO; contract grant number: MAT2010-15982
FCCI Subprogram: Modality ACI-COLABORA Spain-India 2010-2012; contract grant numbers: MAT2010-15310, PRIPIBAR- 2011-1403, MAT2012-38043, and MAT2013-41723-R
JCyL; contract grant numbers: VA049A11, VA152A12, and VA155A12
CIBER-BBN, JCyL, and the Instituto de Salud Carlos III under the “Network Center of Regenerative Medicine and Cellular Therapy of Castilla and Leon” and DST (Indo-Spanish), (New Delhi)
Idioma: eng
URI: http://uvadoc.uva.es/handle/10324/22962
Derechos: info:eu-repo/semantics/openAccess
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