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Título: Human adipose derived stem cells are superior to human osteoblasts (HOB) in bone tissue engineering on a collagen-fibroin-ELR blend
Autor: Sayin, Esen
Hama Rashid, Rosti
Rodríguez Cabello, José Carlos
Elsheikh Erkan, Ahmed
Türker Baran, E.
Hasirci, Vasif
Año del Documento: 2017
Editorial: Royal Society of Chemistry
Descripción: Producción Científica
Documento Fuente: Bioactive Materials, 2017, Volume 2, Issue 2, Pages 71-81
Resumen: The ultrastructure of the bone provides a unique mechanical strength against compressive, torsional and tensional stresses. An elastin-like recombinamer (ELR) with a nucleation sequence for hydroxyapatite was incorporated into films prepared from a collagen – silk fibroin blend carrying microchannel patterns to stimulate anisotropic osteogenesis. SEM and fluorescence microscopy showed the alignment of adipose-derived stem cells (ADSCs) and the human osteoblasts (HOBs) on the ridges and in the grooves of microchannel patterned collagen-fibroin-ELR blend films. The Young's modulus and the ultimate tensile strength (UTS) of untreated films were 0.58 ± 0.13 MPa and 0.18 ± 0.05 MPa, respectively. After 28 days of cell culture, ADSC seeded film had a Young's modulus of 1.21 ± 0.42 MPa and UTS of 0.32 ± 0.15 MPa which were about 3 fold higher than HOB seeded films. The difference in Young's modulus was statistically significant (p: 0.02). ADSCs attached, proliferated and mineralized better than the HOBs. In the light of these results, ADSCs served as a better cell source than HOBs for bone tissue engineering of collagen-fibroin-ELR based constructs used in this study. We have thus shown the enhancement in the tensile mechanical properties of the bone tissue engineered scaffolds by using ADSCs.
Palabras Clave: Células madre
Huesos
Revisión por Pares: SI
DOI: https://doi.org/10.1016/j.bioactmat.2017.04.001
Patrocinador: info:eu-repo/grantAgreement/EC/H2020/642687
info:eu-repo/grantAgreement/EC/H2020/646075
info:eu-repo/grantAgreement/EC/FP7/278557
info:eu-repo/grantAgreement/EC/FP7/317306
Patrocinador: Ministerio de Economía, Industria y Competitividad (Project MAT2013-42473-R and MAT2015-68901R)
Junta de Castilla y León (programa de apoyo a proyectos de investigación – Ref. (VA244U13, VA313U14 and VA015U16)
Version del Editor: http://www.sciencedirect.com/science/article/pii/S2452199X17300038
Idioma: eng
URI: http://uvadoc.uva.es/handle/10324/24424
Derechos: info:eu-repo/semantics/openAccess
Aparece en las colecciones:Documentos OpenAire(Open Access Infrastructure for Research in Europe)
BIOFORGE - Artículos de revista

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