Show simple item record

dc.contributor.authorPescador, David
dc.contributor.authorIbáñez Fonseca, Arturo
dc.contributor.authorSánchez Guijo, Fermín
dc.contributor.authorG. Briñón, Jesús
dc.contributor.authorArias Vallejo, Francisco Javier 
dc.contributor.authorMuntión, Sandra
dc.contributor.authorHernández, Cristina
dc.contributor.authorGirotti, Alessandra
dc.contributor.authorAlonso Rodrigo, Matilde 
dc.contributor.authorCañizo, María Consuelo del
dc.contributor.authorRodríguez Cabello, José Carlos 
dc.contributor.authorBlanco, Juan Francisco
dc.date.accessioned2017-07-14T11:47:22Z
dc.date.available2017-07-14T11:47:22Z
dc.date.issued2017
dc.identifier.citationJournal of Materials Science: Materials in Medicine, 2017, 28:115es
dc.identifier.urihttp://uvadoc.uva.es/handle/10324/24431
dc.descriptionProducción Científicaes
dc.description.abstractOver the last decades, novel therapeutic tools for osteochondral regeneration have arisen from the combination of mesenchymal stromal cells (MSCs) and highly specialized smart biomaterials, such as hydrogel-forming elastin-like recombinamers (ELRs), which could serve as cell-carriers. Herein, we evaluate the delivery of xenogeneic human MSCs (hMSCs) within an injectable ELR-based hydrogel carrier for osteochondral regeneration in rabbits. First, a critical-size osteochondral defect was created in the femora of the animals and subsequently filled with the ELR-based hydrogel alone or with embedded hMSCs. Regeneration outcomes were evaluated after three months by gross assessment, magnetic resonance imaging and computed tomography, showing complete filling of the defect and the de novo formation of hyaline-like cartilage and subchondral bone in the hMSC-treated knees. Furthermore, histological sectioning and staining of every sample confirmed regeneration of the full cartilage thickness and early subchondral bone repair, which was more similar to the native cartilage in the case of the cell-loaded ELR-based hydrogel. Overall histological differences between the two groups were assessed semi-quantitatively using the Wakitani scale and found to be statistically significant (p < 0.05). Immunofluorescence against a human mitochondrial antibody three months post-implantation showed that the hMSCs were integrated into the de novo formed tissue, thus suggesting their ability to overcome the interspecies barrier. Hence, we conclude that the use of xenogeneic MSCs embedded in an ELR-based hydrogel leads to the successful regeneration of hyaline cartilage in osteochondral lesions.es
dc.format.mimetypeapplication/pdfes
dc.language.isoenges
dc.publisherSpringeres
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.classificationCartílagoes
dc.titleRegeneration of hyaline cartilage promoted by xenogeneic mesenchymal stromal cells embedded within elastin-like recombinamer-based bioactive hydrogelses
dc.typeinfo:eu-repo/semantics/articlees
dc.identifier.doi10.1007/s10856-017-5928-1es
dc.relation.publisherversionhttps://rd.springer.com/article/10.1007/s10856-017-5928-1es
dc.peerreviewedSIes
dc.description.projectMinisterio de Economía, Industria y Competitividad (Project (MAT2016-78903-R, MAT2016-9 79435-R, MAT2013-42473-R, MAT2013-41723-R and MAT2012-38043)es
dc.description.projectJunta de Castilla y León (programa de apoyo a proyectos de investigación – Ref. VA244U13, VA313U14 and GRS/516/A/10)es
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/H2020/642687
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/H2020/646075
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/278557
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/317306
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International


Files in this item

Thumbnail
Thumbnail

This item appears in the following Collection(s)

Show simple item record