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Título: Regeneration of hyaline cartilage promoted by xenogeneic mesenchymal stromal cells embedded within elastin-like recombinamer-based bioactive hydrogels
Autor: Pescador, David
Ibáñez Fonseca, Arturo
Sánchez Guijo, Fermín
G. Briñón, Jesús
Arias, Francisco Javier
Muntión, Sandra
Hernández, Cristina
Girotti, Alessandra
Alonso Rodrigo, Matilde
Cañizo, María Consuelo del
Rodríguez Cabello, José Carlos
Blanco, Juan Francisco
Año del Documento: 2017
Editorial: Springer
Descripción: Producción Científica
Documento Fuente: Journal of Materials Science: Materials in Medicine, 2017, 28:115
Resumen: Over the last decades, novel therapeutic tools for osteochondral regeneration have arisen from the combination of mesenchymal stromal cells (MSCs) and highly specialized smart biomaterials, such as hydrogel-forming elastin-like recombinamers (ELRs), which could serve as cell-carriers. Herein, we evaluate the delivery of xenogeneic human MSCs (hMSCs) within an injectable ELR-based hydrogel carrier for osteochondral regeneration in rabbits. First, a critical-size osteochondral defect was created in the femora of the animals and subsequently filled with the ELR-based hydrogel alone or with embedded hMSCs. Regeneration outcomes were evaluated after three months by gross assessment, magnetic resonance imaging and computed tomography, showing complete filling of the defect and the de novo formation of hyaline-like cartilage and subchondral bone in the hMSC-treated knees. Furthermore, histological sectioning and staining of every sample confirmed regeneration of the full cartilage thickness and early subchondral bone repair, which was more similar to the native cartilage in the case of the cell-loaded ELR-based hydrogel. Overall histological differences between the two groups were assessed semi-quantitatively using the Wakitani scale and found to be statistically significant (p < 0.05). Immunofluorescence against a human mitochondrial antibody three months post-implantation showed that the hMSCs were integrated into the de novo formed tissue, thus suggesting their ability to overcome the interspecies barrier. Hence, we conclude that the use of xenogeneic MSCs embedded in an ELR-based hydrogel leads to the successful regeneration of hyaline cartilage in osteochondral lesions.
Palabras Clave: Cartílago
Revisión por Pares: SI
DOI: 10.1007/s10856-017-5928-1
Patrocinador: info:eu-repo/grantAgreement/EC/H2020/642687
Patrocinador: Ministerio de Economía, Industria y Competitividad (Project (MAT2016-78903-R, MAT2016-9 79435-R, MAT2013-42473-R, MAT2013-41723-R and MAT2012-38043)
Junta de Castilla y León (programa de apoyo a proyectos de investigación – Ref. VA244U13, VA313U14 and GRS/516/A/10)
Version del Editor:
Idioma: eng
Derechos: info:eu-repo/semantics/openAccess
Aparece en las colecciones:Documentos OpenAire(Open Access Infrastructure for Research in Europe)
BIOFORGE - Artículos de revista

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