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Título: Control of angiogenesis and host response by modulating the cell adhesion properties of an Elastin-Like Recombinamer-based hydrogel
Autor: Staubli, Sebastian Manuel
Cerino, Giulia
Gonzalez de Torre, Israel
Alonso Rodrigo, Matilde
Oertli, Daniel
Eckstein, Friedrich
Glatz, Katharina
Rodríguez Cabello, José Carlos
Marsano, Anna
Año del Documento: 2017
Editorial: Elsevier
Descripción: Producción Científica
Documento Fuente: Biomaterials Volume 135, August 2017, Pages 30-41
Resumen: The control of the in vivo vascularization of engineered tissue substitutes is essential in order to obtain either a rapid induction or a complete inhibition of the process (e.g. in muscles and hyaline-cartilage, respectively). Among the several polymers available, Elastin-Like Recombinamers (ELR)-based hydrogel stands out as a promising material for tissue engineering thanks to its viscoelastic properties, non-toxicity, and non-immunogenicity. In this study, we hypothesized that varying the cell adhesion properties of ELR-hydrogels could modulate the high angiogenic potential of adipose tissue-derived stromal vascular fraction (SVF) cells, predominantly composed of endothelial/mural and mesenchymal cells. Human SVF cells, embedded in RGD-REDV-bioactivated or unmodified ELR-hydrogels, were implanted in rat subcutaneous pockets either immediately or upon 5-day-culture in perfusion-bioreactors. Perfusion-based culture enhanced the endothelial cell cord-like-organization and the release of pro-angiogenic factors in functionalized constructs. While in vivo vascularization and host cell infiltration within the bioactivated gels were highly enhanced, the two processes were strongly inhibited in non-functionalized SVF-based hydrogels up to 28 days. ELR-based hydrogels showed a great potential to determine the successful integration of engineered substitutes thanks to their capacity to finely control the angiogenic/inflammation process at the recipient site, even in presence of SVF cells.
Palabras Clave: Angiogénesis
Revisión por Pares: SI
DOI: 10.1016/j.biomaterials.2017.04.047
Patrocinador: info:eu-repo/grantAgreement/EC/H2020/642687
info:eu-repo/grantAgreement/EC/FP//278557
info:eu-repo/grantAgreement/EC/H2020/646075
info:eu-repo/grantAgreement/EC/FP7/317304
Patrocinador: Ministerio de Economía, Industria y Competitividad (Project MAT-2010-15982, MAT2010- 15310, PRI-PIBAR-2011-1403 and MAT2012-38043-C02-01)
Junta de Castilla y León (programa de apoyo a proyectos de investigación – Ref.VA152A12-2, VA244U13 and VA155A12-2)
Version del Editor: http://www.sciencedirect.com/science/article/pii/S0142961217302843
Idioma: eng
URI: http://uvadoc.uva.es/handle/10324/24758
Derechos: info:eu-repo/semantics/openAccess
Aparece en las colecciones:Documentos OpenAire(Open Access Infrastructure for Research in Europe)
BIOFORGE - Artículos de revista

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