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Título: Bone regeneration mediated by a bioactive and biodegradable ECM-like hydrogel based on elastin-like recombinamers
Autor: Coletta, Dante J.
Ibáñez Fonseca, Arturo
Missana, Liliana R.
Jammal, María V.
Vitelli, Ezequiel J.
Aimone, Mariangeles
Zabalza, Facundo
Issa, João P. Mardegan
Alonso Rodrigo, Matilde
Rodríguez Cabello, José Carlos
Feldman, Sara
Año del Documento: 2017
Editorial: Mary Ann Liebert
Descripción: Producción Científica
Documento Fuente: Tissue engineering. Part A, 2017
Resumen: The morbidity of bone fractures and defects is steadily increasing due to changes in the age pyramid. As such, novel biomaterials that are able to promote the healing and regeneration of injured bones are needed in order to overcome the limitations of auto-, allo-, and xenografts, while providing a ready-to-use product that may help to minimize surgical invasiveness and duration. In this regard, recombinant biomaterials, such as elastin-like recombinamers (ELRs), are very promising as their design can be tailored by genetic engineering, thus allowing scalable production and batch-to-batch consistency, amongst others. Furthermore, they can self-assemble into physically cross-linked hydrogels above a certain transition temperature, in this case body temperature, but are injectable below this temperature, thereby markedly reducing surgical invasiveness. Herein we have developed two bioactive hydrogel-forming ELRs, one including the osteogenic and osteoinductive BMP-2 and the other the RGD cell-adhesion motif. The combination of these two novel ELRs results in a BMP-2-loaded extracellular matrix-like hydrogel. Moreover, elastase-sensitive domains were included in both ELR molecules, thereby conferring biodegradation as a result of enzymatic cleavage and avoiding the need for scaffold removal after bone regeneration. Both ELRs and their combination showed excellent cytocompatibility, and the culture of cells on RGD-containing ELRs resulted in optimal cell adhesion. In addition, hydrogels based on a mixture of both ELRs were implanted in a pilot study involving a femoral bone injury model in New Zealand White rabbits, showing complete regeneration in six out of seven cases, with the other showing partial closure of the defect. Moreover, bone neo-formation was confirmed using different techniques, such as radiography, computed tomography and histology. This hydrogel system therefore displays significant potential in the regeneration of bone defects, promoting self-regeneration by the surrounding tissue with no involvement of stem cells or osteogenic factors other than BMP-2, which is released in a controlled manner by elastase-mediated cleavage from the ELR backbone.
Palabras Clave: Regeneración ósea
Revisión por Pares: SI
Patrocinador: Ministerio de Economía, Industria y Competitividad (Project (MAT2013-42473-R and MAT2013-41723-R)
Junta de Castilla y León (programa de apoyo a proyectos de investigación – Ref. VA244U13 and VA313U14)
Centro en Red de Medicina Regenerativa y Terapia Celular de Castilla y León
Comisión Europea (proyectos NMP-2014-646075, HEALTH-F4-2011-278557, PITN-GA-2012-317306 y MSCA-ITN-2014-642687)
Idioma: eng
Derechos: info:eu-repo/semantics/openAccess
Aparece en las colecciones:BIOFORGE - Artículos de revista

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