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Por favor, use este identificador para citar o enlazar este ítem: http://uvadoc.uva.es/handle/10324/24816
Título: Gelatin-Hyaluronic Acid Hydrogels with Tuned Stiffness to Counterbalance Cellular Forces and Promote Cell Differentiation
Autor: Poveda Reyes, Sara
Moulisova, Vladimira
Sanmartín Masiá, Esther
Quintanilla Sierra, Luis
Salmerón Sánchez, Manuel
Gallego Ferrer, Gloria
Año del Documento: 2016
Editorial: Wiley
Descripción: Producción Científica
Documento Fuente: Macromolecular Bioscience, 2016, 16(9), pg. 1311-24
Resumen: Cells interact mechanically with their environment, exerting mechanical forces that probe the extracellular matrix (ECM). The mechanical properties of the ECM determine cell behavior and control cell differentiation both in 2D and 3D environments. Gelatin (Gel) is a soft hydrogel into which cells can be embedded. This study shows significant 3D Gel shrinking due to the high traction cellular forces exerted by the cells on the matrix, which prevents cell differentiation. To modulate this process, Gel with hyaluronic acid (HA) has been combined in an injectable crosslinked hydrogel with controlled Gel–HA ratio. HA increases matrix stiffness. The addition of small amounts of HA leads to a significant reduction in hydrogel shrinking after cell encapsulation (C2C12 myoblasts). We show that hydrogel stiffness counterbalanced traction forces of cells and this was decisive in promoting cell differentiation and myotube formation of C2C12 encapsulated in the hybrid hydrogels.
Palabras Clave: Gelatina
Ácido hialurónico
Revisión por Pares: SI
DOI: 10.1002/mabi.201500469
Patrocinador: Ministerio de Economía, Industria y Competitividad (MAT2013-46467-C4-1-R)
CIBER-BBN y el Instituto de Salud Carlos III mediante el Centro de Medicina Regenerativa y Terapia Celular de Castilla y León
Version del Editor: http://onlinelibrary.wiley.com/doi/10.1002/mabi.201500469/abstract
Idioma: eng
URI: http://uvadoc.uva.es/handle/10324/24816
Derechos: info:eu-repo/semantics/openAccess
Embargado hasta2018-01-01
Aparece en las colecciones:BIOFORGE - Artículos de revista

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