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Title: Gelatin-Hyaluronic Acid Hydrogels with Tuned Stiffness to Counterbalance Cellular Forces and Promote Cell Differentiation
Authors: Poveda Reyes, Sara
Moulisova, Vladimira
Sanmartín Masiá, Esther
Quintanilla Sierra, Luis
Salmerón Sánchez, Manuel
Gallego Ferrer, Gloria
Issue Date: 2016
Publisher: Wiley
Description: Producción Científica
Citation: Macromolecular Bioscience, 2016, 16(9), pg. 1311-24
Abstract: Cells interact mechanically with their environment, exerting mechanical forces that probe the extracellular matrix (ECM). The mechanical properties of the ECM determine cell behavior and control cell differentiation both in 2D and 3D environments. Gelatin (Gel) is a soft hydrogel into which cells can be embedded. This study shows significant 3D Gel shrinking due to the high traction cellular forces exerted by the cells on the matrix, which prevents cell differentiation. To modulate this process, Gel with hyaluronic acid (HA) has been combined in an injectable crosslinked hydrogel with controlled Gel–HA ratio. HA increases matrix stiffness. The addition of small amounts of HA leads to a significant reduction in hydrogel shrinking after cell encapsulation (C2C12 myoblasts). We show that hydrogel stiffness counterbalanced traction forces of cells and this was decisive in promoting cell differentiation and myotube formation of C2C12 encapsulated in the hybrid hydrogels.
Classification: Gelatina
Ácido hialurónico
Peer Review: SI
DOI: 10.1002/mabi.201500469
Sponsor: Ministerio de Economía, Industria y Competitividad (MAT2013-46467-C4-1-R)
CIBER-BBN y el Instituto de Salud Carlos III mediante el Centro de Medicina Regenerativa y Terapia Celular de Castilla y León
Publisher Version:
Language: eng
Rights: info:eu-repo/semantics/openAccess
Appears in Collections:BIOFORGE - Artículos de revista

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