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Title: Biocompatibility of two model elastin‐like recombinamer‐based hydrogels formed through physical or chemical cross‐linking for various applications in tissue engineering and regenerative medicine
Authors: Ibáñez Fonseca, Arturo
Ramos, Teresa L.
González de Torre, Israel
Sánchez Abarca, Luis Ignacio
Muntión, Sandra
Arias Vallejo, F. J.
Cañizo, María Consuelo del
Alonso Rodrigo, Matilde
Sánchez Guijo, Fermín
Rodríguez Cabello, José Carlos
Issue Date: 2018
Publisher: Wiley
Description: Producción Científica
Citation: Journal of Tissue Engineering and Regenerative Medicine, 2018, Volume12, Issue3, Pages e1450-e1460
Abstract: Biocompatibility studies, especially innate immunity induction, in vitro and in vivo cytotoxicity, and fibrosis, are often lacking for many novel biomaterials including recombinant protein‐based ones, such as elastin‐like recombinamers (ELRs), and has not been extensively explored in the scientific literature, in contrast to traditional biomaterials. Herein, we present the results from a set of experiments designed to elucidate the preliminary biocompatibility of 2 types of ELRs that are able to form extracellular matrix‐like hydrogels through either physical or chemical cross‐linking both of which are intended for different applications in tissue engineering and regenerative medicine. Initially, we present in vitro cytocompatibility results obtained upon culturing human umbilical vein endothelial cells on ELR substrates, showing optimal proliferation up to 9 days. Regarding in vivo cytocompatibility, luciferase‐expressing hMSCs were viable for at least 4 weeks in terms of bioluminescence emission when embedded in ELR hydrogels and injected subcutaneously into immunosuppressed mice. Furthermore, both types of ELR‐based hydrogels were injected subcutaneously in immunocompetent mice and serum TNFα, IL‐1β, IL‐4, IL‐6, and IL‐10 concentrations were measured by enzyme‐linked immunosorbent assay, confirming the lack of inflammatory response, as also observed upon macroscopic and histological evaluation. All these findings suggest that both types of ELRs possess broad biocompatibility, thus making them very promising for tissue engineering and regenerative medicine‐related applications.
Classification: Ingeniería de tejidos
Medicina regenerativa
Tissue engineering
Regenerative medicine
ISSN: 1932-6254
Peer Review: SI
Sponsor: European Commission (NMP-2014-646075, HEALTH-F4-2011-278557, PITN-GA-2012-317306 and MSCA-ITN-2014-642687)
Ministerio de Economía, Industria y Competitividad (Projects MAT2016-78903-R, MAT2016-79435-R, MAT2013-42473-R, MAT2013-41723-R and MAT2012-38043)
Junta de Castilla y León (programa de apoyo a proyectos de investigación – Ref.VA244U13 and VA313U14)
Centro en Red de Medicina Regenerativa y Terapia Celular de Castilla y León
Instituto de Salud Carlos III (grant RD12/0019/0017 )
Fundação para a Ciência e Tecnologia (SFRH/BD/86451/ 2012)
Publisher Version:
Language: eng
Rights: info:eu-repo/semantics/openAccess
Appears in Collections:BIOFORGE - Artículos de revista

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